Laboratory Handbook on Biochemistry
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Laboratory Handbook on Biochemistry

Laboratory Handbook on Biochemistry


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About the Book

Table of Contents:
Contents; Foreword; Preface; Acknowledgements; Section One: AMINO ACIDS, PROTEINS, ENZYMES AND OTHER NITROGENOUS COMPOUNDS; 1. Qualitative Analysis of Amino Acids--General Procedure; 2. Estimation of Glycine by Ninhydrin Method; 3. Estimation of Proline by Ninhydrin Method; 4. Qualitative Analysis of Proteins 5. Estimation of Total Proteins by Folin-Lowry's Method 6. Estimation of Total Proteins by Biuret Method; 7. Estimation of Proteins by Bradford Method; 8. Estimation of Proteins by Copper (II)--Neocuproine Method; 9. Estimation of Proteins by Warburg-Christian Assay (Spectrophotometric Assay at 280 nm); 10. Estimation of Albumin by Bromocresol Green (BCG) Method; 11. Estimation of Creatinine by Alkaline Picrate (Jaffe's) Method; 12. Effect of pH on the Rate of Acid Phosphatase Catalyzed Reaction; 13. Effect of Temperature on the Rate of Acid Phosphatase Catalyzed Reaction; 14. Estimation of Urea by Diacetyl Monoxime-Thiosemicarbazide (DAM-TSC) Method; 15. Estimation of Uric Acid by Henry Caraway Method; Section Two: CARBOHYDRATES; 16. Qualitative Analysis of Carbohydrates--General Procedure; 17. Estimation of Free Reducing Sugars by 3, 5-Dinitrosalicylic Acid Method; 18. Estimation of Glucose by O-toluidine Method; 19. Estimation of Xylose by Orcinol Method; 20. Estimation of Fructose by Resorcinol Method; 21. Hydrolysis of Starch and Estimation of Glucose by Glucose Oxidase and Peroxidase (GOD-POD) Method; Section Three: LIPIDS; 22. Qualitative Analysis of Lipids; 23. Estimation of Cholesterol by Zak's Method; 24. Estimation of Sterols by Liebermann-Burchard Method; Section Four: NUCLEIC ACID; 25. Estimation of DNA by Diphenylamine Method; 26. Estimation of DNA by UV Spectrophotometry; 27. Estimation of RNA by Orcinol Method; Section Five: ALKALOIDS, FLAVONOIDS, PHENOLIC COMPOUNDS, VITAMINS, TANNINS; 28. Estimation of Alkaloids by Singh and Archana Sahu Method; 29. Estimation of Flavonoids by Aluminium Chloride Method (Zhishen Mengcheng, and Jianming); 30. Estimation of Total Phenolics by Modified Folin-Ciocalteau Method (Eberhardt et al.); 31. Estimation of Ascorbic Acid (Vitamin C) by 2, 4-DNPH Method; 32. Estimation of Vitamin E by Baker and Frank Method; 33. Estimation of Tannins by Modified Prussian Blue Method; Section Six: INVITRO ANTIOXIDANT ASSAY; 34. Ferric Ion Reducing/Antioxidant Power (FRAP) Assay (Oyaizu Method); 35. Modified Ferric Ion Reducing/Antioxidant Power Assay (Modified FRAP Assay); 36. Modified CUPRAC (Cupric Ion Reducing Antioxidant Capacity) Assay (Resat Apak et al.); 37. Ferrous Ion Chelating Ability Assay (Decker and Welch Method); 38. Determination of Total Antioxidant Capacity by Phosphomolybdenum Method (Prieto et al.); 39. Modified Hydroxyl Radical Scavenging Assay (Resat Apak et al.); 40. ABTS Radical Scavenging Assay (Re et al.); 41. DPPH Radical Scavenging Assay (Shimada et al.); 42. Phytochemical Screening; Appendix I Preparation of Laboratory Reagents: Carbohydrates-Qualitative; Appendix II Preparation of Laboratory Reagents: Amino Acids & Proteins-Qualitative; Appendix III Definitions; Index.

About the Author :
K. PANNEER SELVAM (Ph.D.) is Associate Professor in the Department of Microbiology, Dr. G.R. Damodaran College of Science, Coimbatore. He has published several peer reviewed research papers in various National and International journals and conducted many DBT/DST sponsored state level, National and International seminars and training programmes. Dr. Panneer Selvam's areas of research interest include medical microbiology, medical biotechnology and recombinant DNA technology. T. SATHISH KUMAR is Senior Lecturer in the Department of Biotechnology, Kumaraguru College of Technology, Coimbatore. With more than 11 years of teaching experience, he has published more than 18 peer reviewed research papers in various National and International journals. His area of research interest is molecular modelling of proteins. S. SHANMUGAM (Ph.D.) is Assistant Professor in the Department of Biotechnology, Kumaraguru College of Technology, Coimbatore. With nearly 15 years of teaching experience, he has published more than 20 peer reviewed research papers in various National and International journals. Dr. Shanmugam's areas of research interest include enzymology and immunology.


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Product Details
  • ISBN-13: 9788120341036
  • Publisher: Prentice-Hall of India Pvt.Ltd
  • Publisher Imprint: Prentice-Hall of India Pvt.Ltd
  • Height: 235 mm
  • No of Pages: 152
  • Width: 178 mm
  • ISBN-10: 8120341031
  • Publisher Date: 01 Aug 2010
  • Binding: Paperback
  • Language: English
  • Weight: 260 gr


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