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Potenziamento dei principi attivi da parte di A. rhizogenes in Hyoscyamus muticus

Potenziamento dei principi attivi da parte di A. rhizogenes in Hyoscyamus muticus


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Nella prima parte di questo studio, il protocollo ottimizzato per l'induzione delle radici pelose dell'hyoscyamus muticus consiste nell'utilizzare espianti di foglie di 2 mesi di età infettati con il ceppo LBA 9402/12 di A. rhizogenes a un'intensità corrispondente a OD600 = 0,6, utilizzando il metodo di infezione mediante immersione normale per 30 minuti in combinazione con acetosiringone (500 μM). Gli espianti fogliari sono stati incubati su terreno MS per 2 giorni in condizioni di oscurità. Successivamente, sono stati trasferiti su terreno MS contenente 500 mg/l di rifampicina e incubati per 16 giorni in condizioni di 16/8 ore (giorno/notte). Gli espianti fogliari infettati con LBA 9402/12 sono stati coltivati su MS e incubati a 25 °C in condizioni di giorno/notte per 16 giorni, producendo una risposta significativamente più elevata per le radici pelose e presentando la curva di crescita più lunga nello studio cinetico della crescita. Nella seconda parte, il peso fresco del callo più elevato è stato ottenuto dagli espianti di foglie di H. muticus coltivati su MS contenente 0,5 mg/l di BAP combinato con 1 mg/l di 2,4-D e incubati per 4 settimane a 25 °C in condizioni di giorno/notte. Nella terza parte, il clone di radici pelose trasformato indotto dal ceppo LBA 9402/12 ha mostrato le concentrazioni più elevate di iosciamina e scopolamina rispetto alla concentrazione delle altre parti della pianta o del callo indotto.


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Product Details
  • ISBN-13: 9786203892482
  • Publisher: International Book Market Service Ltd
  • Publisher Imprint: International Book Market Service Ltd
  • Height: 229 mm
  • No of Pages: 132
  • Returnable: N
  • Weight: 240 gr
  • ISBN-10: 6203892483
  • Publisher Date: 22 Apr 2025
  • Binding: Paperback
  • Language: Italian
  • Returnable: N
  • Spine Width: 8 mm
  • Width: 152 mm


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