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Verstärkung der Wirkstoffe durch A. rhizogenes in Hyoscyamus muticus

Verstärkung der Wirkstoffe durch A. rhizogenes in Hyoscyamus muticus


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Im ersten Teil dieser Studie wurde das optimierte Protokoll zur Induktion von Haarwurzeln bei ägyptischem Bilsenkraut entwickelt. Dazu wurden zwei Monate alte, herausgeschnittene Blattexplantate verwendet, die mit dem Stamm LBA 9402/12 von A. rhizogenes in einer Intensität entsprechend OD600 = 0,6 infiziert wurden. Die Infektion erfolgte durch 30-minütiges Eintauchen in eine Lösung mit Acetosyringon (500 μM). Die Blattexplantate wurden 2 Tage lang unter dunklen Bedingungen auf MS-Medium inkubiert. Anschließend wurden sie in MS-Medium mit 500 mg/l Rifampicin überführt und 16 Tage lang unter 16/8 h (Tag/Nacht) inkubiert. Mit LBA 9402/12 infizierte Blattexplantate wurden auf MS kultiviert und 16 Tage lang bei Tag/Nacht-Bedingungen bei 25 °C inkubiert, was zu einer deutlich höheren Reaktion für Haarwurzeln führte und die längste Wachstumskurve in der Wachstumskinetikstudie aufwies. Im zweiten Teil wurde das höchste Frischgewicht des Kallus aus H. muticus-Blattexplantaten gewonnen, die auf MS mit 0,5 mg/l BAP in Kombination mit 1 mg/l 2,4-D kultiviert und 4 Wochen lang bei 25 °C unter Tag-/Nachtbedingungen inkubiert wurden. Im dritten Teil wies der aus dem Stamm LBA 9402/12 induzierte transformierte Haarwurzelklon im Vergleich zu den Konzentrationen in anderen Pflanzenteilen oder induziertem Kallus die höchsten Konzentrationen an Hyoscyamin und Scopolamin auf.


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Product Details
  • ISBN-13: 9786203892451
  • Publisher: International Book Market Service Ltd
  • Publisher Imprint: International Book Market Service Ltd
  • Height: 229 mm
  • No of Pages: 140
  • Spine Width: 8 mm
  • Width: 152 mm
  • ISBN-10: 6203892459
  • Publisher Date: 22 Apr 2025
  • Binding: Paperback
  • Language: German
  • Returnable: N
  • Weight: 249 gr


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