Laboratory Techniques in Renal Cell and Molecular Biology
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Home > Medicine & Health Science textbooks > Clinical and internal medicine > Renal medicine and nephrology > Laboratory Techniques in Renal Cell and Molecular Biology
Laboratory Techniques in Renal Cell and Molecular Biology

Laboratory Techniques in Renal Cell and Molecular Biology


     0     
5
4
3
2
1



Out of Stock


Notify me when this book is in stock
X
About the Book

Research on renal diseases is progressing at a rapid pace, bringing with it a major change in the research techniques used: There has been a shift from urine and blood analysis or histological examinations by light microscopy to immunohistochemical assays at the protein levels, and studies are now being performed at the molecular and gene levels. Keeping up with this rapid development in the field of new molecular biology techniques is not always easy. It is exactly at this point that the book at hand comes into the picture: Meant as a research manual, it provides a concise outline of the objectives, principles and procedures of the tests currently available. Each step is meticulously described, guiding the researcher through the different stages of an assay. One of the special features of this publication is the list of pitfalls which researchers, in particular those just starting out in this field, might face. As the research pursued at present covers a wide range of topics, specialists in each field were asked to contribute to the corresponding sections. This book has been especially written for specialists in the fields of nephrology, urology, pediatrics, immunology and pathology who are new to research on kidney disease.

Table of Contents:
Part 1 Specimen preparation and storage procedures: fluorescence antibody method; in situ hybridization; enzyme antibody method; electron microscopy (TEM, SEM, immunoelectron microscopy); isolation of peripheral mononuclear cells; isolation of glomeruli; RNA extraction (renal tissue, glomeruli, mononuclear and other cells, etc; DNA extraction (renal tissue, glomeruli, mononuclear and other cells, etc); preparation of cDNA libraries; cell culture methods - mesangial cells; cell culture methods -epithelian and endothelial cells; cell culture methods - renal tubular cells. Part 2 Protein and gene procedures: fluorescent antibody method - renal tissue; fluorescent antibody method -lymphocyte; analysis by flow cytometry; enzyme antibody method; electron microscopy (TEM, SEM and immunoelectron microscopy); western blot method; immunoprecipitation methods; northern blot method; dot blot method (slot blot method); southern blot method; polymerase chain reaction (PCR); reverse transcription (RT)-PCR method; in situ PCR; RFLP analysis; microsatellite method; in situ hybridization. Part 3 Culture cell procedures: cell proliferation; intracellular signal transmission; gene cloning method by ddPCR (differential display polymerase chain reaction).


Best Sellers


Product Details
  • ISBN-13: 9783805569989
  • Publisher: S Karger Ag
  • Publisher Imprint: S Karger AG
  • Language: English
  • Weight: 480 gr
  • ISBN-10: 380556998X
  • Publisher Date: 26 Jan 2000
  • Binding: Spiral bound
  • No of Pages: 210


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
Laboratory Techniques in Renal Cell and Molecular Biology
S Karger Ag -
Laboratory Techniques in Renal Cell and Molecular Biology
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

Laboratory Techniques in Renal Cell and Molecular Biology

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept

    Fresh on the Shelf


    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!