Laboratory Techniques in Biochemistry and Molecular Biology
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Laboratory Techniques in Biochemistry and Molecular Biology: v. 9(Laboratory Techniques in Biochemistry and Molecular Biology)

Laboratory Techniques in Biochemistry and Molecular Biology: v. 9(Laboratory Techniques in Biochemistry and Molecular Biology)

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About the Book

Although there is a continuing high level of activity in direct studies on protein primary structures, the requirement for partial sequences has provided a major impetus for the development and dissemination of highly sensitive techniques. While retaining the still applicable formidable basis of the original volume, this Revised Edition reflects the shift towards the use of more sophisticated automated high-performance chromatographic techniques. Sequencing of Proteins and Peptides provides you with the most up-to-date information selected to be the most appropriate for laboratory or reference use since, as in the first edition, it is impossible to cover the field of protein sequence determination fully. New information covers: #FABMS #protein recovery methods #Amino acid analysis #Tandem, plasma description, FAB, and Thermospray mass spectrometry. As in the First edition, Appendixes give information on manufacturers and supplies, purification, and safety in the lab.

Table of Contents:
Preface. List of Abbreviations. Chapter headings: 1. Introduction. 2. Preliminary characterization of the protein. 3. Specific cleavage of the protein. 4. Separation and purification of peptides. 5. Methods for the detection of peptides. 6. Determination of peptide sequences. 7. Structures introduced by post-translational modification. 8. Deduction of the total primary structure of a protein from the sequences of constituent peptides. 9. Integral membrane proteins. 10. Applications of partial sequence analysis and relationship to DNA sequencing. Appendix I. Manufacturers and supplies of equipment and reagents for protein sequence analysis. II. Purification of solvents and reagents. III. Safety in the protein chemistry laboratory. IV. Notes on work with very small amounts of proteins and peptides. References. Subject index.


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Product Details
  • ISBN-13: 9780444810229
  • Publisher: Elsevier Science & Technology
  • Publisher Imprint: Elsevier Science Ltd
  • Edition: Revised edition
  • Language: English
  • Sub Title: v. 9
  • ISBN-10: 0444810226
  • Publisher Date: /02/1989
  • Binding: Paperback
  • Height: 200 mm
  • Series Title: Laboratory Techniques in Biochemistry and Molecular Biology


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Laboratory Techniques in Biochemistry and Molecular Biology: v. 9(Laboratory Techniques in Biochemistry and Molecular Biology)
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