Rapid Cycle Real-Time PCR — Methods and Applications
Home > Medicine & Health Science textbooks > Clinical and internal medicine > Diseases and disorders > Oncology > Rapid Cycle Real-Time PCR — Methods and Applications: Genetics and Oncology
Rapid Cycle Real-Time PCR — Methods and Applications: Genetics and Oncology

Rapid Cycle Real-Time PCR — Methods and Applications: Genetics and Oncology


     0     
5
4
3
2
1



Out of Stock


Notify me when this book is in stock
X
About the Book

Rapid-Cycle Real-Time PCR is a powerful technique for nucleic acid amplification and analysis that often requires less than half an hour to perform. Samples are amplified by rapid-cycle PCR followed by immediate melting curve analysis in the same instrument. Melting curve analysis of PCR products with SYBR Green I often allows product identification without gel electrophoresis. Furthermore, in the presence of fluorescent hybridization probes, melting curves provide "dynamic dot blots" for fine sequence analysis, including single nucleotide polymorphisms (SNPs). The method is often cited as the most versatile, efficient method for nucleic acid analysis in research and diagnostics in the fields of genetics and oncology. Molecular diagnostics has never been easier!

Table of Contents:
for Genetics and Oncology Volume Rapid Cycle Real-Time PCR Methods and Applications.- I Methods Useful in Genetics and Oncology.- Quantification of Cytokine mRNAs in Human Myocardial Biopsy Samples by Real-Time Quantitative PCR Technology Using the LightCycler Instrument.- Quantitative Two-Step RT-PCR for the Detection of Human ABCA1 Transporter on LightCycler Using Hybridization Probes and External Standards.- Quantification of Human Genomic DNA Using Retinoic X Receptor B Gene.- Genotyping by Guanosine-Dependent Quenching of Single-Labeled Fluorescein Probes.- Limitations of Melting Curve Analysis Using SYBR Green I — Fragment Differentiation and Mutation Detection in the CFTR-Gene.- SYBR Green I Analysis of the Trinucleotide Repeat Responsible for Huntington’s Disease.- II Applications in Genetics.- Parallel Genotyping of Different Genes: A Rapid Real-Time PCR Approach.- Detection of a Single Base Substitution in Single Cells by Melting Peak Analysis Using Dual-Color Hybridization Probes.- Rapid Screening for Five Major Cystic Fibrosis Mutations by Melting Peak Analysis Using Fluorogenic Hybridization Probes.- LightCycler PCR for the Polymorphisms —308 and —238 in the TNF AIpha Gene and for the TNFB1/B2 Polymorphism in the LT Alpha Gene.- Rapid Genotyping of 2-bp and 9-bp Deletion Mutations Using the LightCycler.- Genotyping of the Methionine-Valine Polymorphism at Codon 129 of the Human Prion Protein by Melting Point Analysis of Fluorescently Labeled Hybridization Probes.- Rapid Detection of Missense Mutations in the Prostatic Steroid 5?-Reductase Gene Using Real-Time Fluorescence PCR and Melting Curve Analysis.- III Applications in Oncolon.- Analysis of Microsatellite Instability by Melting Peak Analysis with BAT26 and BAT25 Specific FluorescenceHybridization Probes.- Two Color Multiplexing and Typing of Human Papillomavirus Types 16,18 and 45 on LightCycler.- Quantitative Analysis of AML1-ETO Fusion Transcripts in t(8;21) Positive AML Using Real-Time RT-PCR.- Rapid Quantitative Detection of Free Cancer Cells in the Peritoneal Cavity of Gastric Cancer Patients with Real-Time CEA RT-PCR Using Hybridization Probes.- Quantitative Measurement of the mRNA Expression of the Tumor-Associated Antigen PRAME by Real-Time RT-PCR Using LightCycler and SYBR Green I Technology.- Expression Analysis of Telomerase-Genes hTERT and hTR by Quantitative PCR on LightCycler.- Measurement of MDR1 Gene Expression by Real-Time Quantitative RT-PCR Using the LightCycler Instrument.


Best Sellers


Product Details
  • ISBN-13: 9783642639654
  • Publisher: Springer-Verlag Berlin and Heidelberg GmbH & Co. KG
  • Publisher Imprint: Springer-Verlag Berlin and Heidelberg GmbH & Co. K
  • Height: 242 mm
  • No of Pages: 205
  • Sub Title: Genetics and Oncology
  • ISBN-10: 3642639658
  • Publisher Date: 30 Sep 2011
  • Binding: Paperback
  • Language: English
  • Returnable: Y
  • Width: 193 mm


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
Rapid Cycle Real-Time PCR — Methods and Applications: Genetics and Oncology
Springer-Verlag Berlin and Heidelberg GmbH & Co. KG -
Rapid Cycle Real-Time PCR — Methods and Applications: Genetics and Oncology
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

Rapid Cycle Real-Time PCR — Methods and Applications: Genetics and Oncology

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept

    New Arrivals


    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!