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Home > Mathematics and Science Textbooks > Biology, life sciences > Real-time PCR in Practice
Real-time PCR in Practice

Real-time PCR in Practice


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About the Book

For novices as well as experts, this handy benchtop reference covers basic protocols and theoretical background for first-time users, while also addressing advanced methods and strategies for high performance applications. As such, it teaches the basic requirements, practical know-how as well as the hurdles and pitfalls in obtaining meaningful results in readily comprehensible terms. The authors explain the biochemical principles behind this method and discuss examples of its most common applications including an abundance of practical tips and tricks, plus a special troubleshooting section.

Table of Contents:
INTRODUCTION FLUORESCENT CHEMISTRIES double strand sensitive dyes energy transfer probes REACTION SETUP template, reagents, cycling protocols RT-PCR RNA isolation and quality check priming and reverse transcription trouble shooting QUANTITATIVE REAL-TIME PCR principles absolute vs. relative quantification trouble shooting MELTING CURVE ANALYSIS principles product characterization genotyping and mutation detection OPTIMIZATION STRATEGIES INSTRUMENTATION suppliers, instruments, internet resources APPLICATION EXAMPLES mRNA expression gene regulation bacteria and virus detection gene copy number analysis allele frequency genotyping and mutation analysis

About the Author :
Jochen Wilhelm studied biology at the University of Giessen (Germany), where he also obtained his PhD. During his PhD work at the Department of Biochemistry he developed methods, mathematical models and computer software for real-time PCR applications. He is currently heading the microarray core facility at the University Hospital and part of an interdepartmental research program on the cardiovascular system.


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Product Details
  • ISBN-13: 9783527316878
  • Publisher: Wiley-VCH Verlag GmbH
  • Publisher Imprint: Wiley-VCH Verlag GmbH
  • Height: 240 mm
  • ISBN-10: 3527316876
  • Publisher Date: 09 Nov 2015
  • Binding: Hardback
  • Width: 170 mm


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