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Home > Mathematics and Science Textbooks > Biology, life sciences > Biochemistry > Immunochemical Protocols: (295 Methods in Molecular Biology)
Immunochemical Protocols: (295 Methods in Molecular Biology)

Immunochemical Protocols: (295 Methods in Molecular Biology)


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About the Book

This new edition of a much praised classic includes both basic method for producing and using antibodies and advanced techniques required by today's state-of-the art investigators. The basic sections of the book stress the first principles of antibody production and guide the reader through the processes and pitfalls of immunochemistry. The advanced sections focus on specific applications and cover the immunochemical techniques now used in laboratories. New material includes techniques for making new monoclonal cell lines, for producing good quality monoclonal antibodies, immunocapture PCR, immunoaffinity purification, and immunoelectron microscopy in plant virus diagnosis.

Table of Contents:
Immunization Strategies for Antibody Production.- Synthetic Peptides as Antigens for Antibody Production.- Production of Polyclonal Antibodies in Rabbits.- Making Hybridomas.- Growing Hybridomas.- Phage Display Vectors for the In Vitro Generation of Human Antibody Fragments.- Purification of Antibodies and Preparation of Antibody Fragments.- Conjugation of Antibodies to Alkaline Phosphatase.- Conjugation of Antibodies to Horseradish Peroxidase.- Conjugation of Antibodies to Fluorescein or Rhodamine.- Conjugation of Antibodies to Biotin.- Preparation of Colloidal Gold Probes.- Immunogold Probes in Light Microscopy.- Immunoelectron Microscope Techniques in Plant Virus Diagnosis.- Dual-Antibody Sandwich Enzyme-Linked Immunosorbent Assay.- Competitive Enzyme-Linked Immunosorbent Assay.- Immunoblotting Techniques.- Epitope Mapping.- Generation and Testing of Immunoaffinity Columns.- Immunocapture-Polymerase Chain Reaction.- Magnetic Cell Sorting.- Cell Enrichment and Immunochemical Staining.

Review :
Reviews of Previous editions:
"...a treasury of detailed information, both within the protocols and in the Notes, which in some chapters are extensive...will be useful to both those who already have some experience in this area and...to those who wish to embark on new techniques."
-Microbiology Today

An excellent and well arranged compilation of a range of antibody techniques....An excellent laboratory manual.-Immunoassay


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Product Details
  • ISBN-13: 9781617374661
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Edition: Revised edition
  • Language: English
  • Returnable: Y
  • Width: 152 mm
  • ISBN-10: 1617374660
  • Publisher Date: 10 Nov 2010
  • Binding: Paperback
  • Height: 229 mm
  • No of Pages: 318
  • Series Title: 295 Methods in Molecular Biology


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