Immunochemical Protocols: (v. 80 Methods in Molecular Biology)

This much anticipated second edition provides a user-friendly, up-to-date handbook of reliable immunochemical techniques optimized for molecular biologists. It covers the breadth of relevant established methods from protein blotting and immunoassays through to visualization of cellular antigens and in situ hybridization, each with their latest refinements. Protocols for the production and purification of important classes of immunochemical reagents are also provided, including conventional and recombinant antibodies, fusion proteins and their various conjugates. This book will open the door to a new generation of immunochemical reagents with exciting possibilities.

Table of Contents:
Production of Polyclonal AntiseraRaising Polyclonal Antibodies Using Nitrocellulose-Bound AntigenProduction and Characterization of Antibodies Against Synthetic Peptides Preparation and Testing of MAbs to Recombinant Proteins A Simple Hollow-Fiber Bioreactor for the In-House Production of Monoclonal Antibodies. Screening of Monoclonal Antibodies Using Antigens Labeled with AcetylcholinesteraseSynthesis of Peptides for Use as Immunogens Use of Proteins Blotted to Polyvinylidene Difluoride Membranes as Immunogens Purification of Glycoproteins and Their Use as Immunogens .IgG PurificationPurification of Monoclonal Antibodies Production of Bispecific and Trispecific F(ab)2 and F(ab)3 Antibody DerivativesPreparation of Cytotoxic Antibody & Toxin Conjugates Immunoafunity Puriucation and Quantiucation of Antibody Toxin Conjugates Competitive ELISA Kittima Makarananda Epitope Mapping Coupling of Antibodies with Biotin Preparation of Avidin Conjugates Enhanced Chemiluminescence Immunoassay Nonradioactive Methods for Visualization of Protein Blots Colloidal Gold Staining and Immunoprobingon the Same Western BlotErasure of Western Blots After Autoradiographic or Chemiluminescent Detection Colloidal Gold Staining and Immunodetection in 2D Protein Mapping Enzyme Antienzyme Method for Immunohistochemistry Immunohistochemical Detection of Bromodeoxyuridine-Labeled Nuclei for In Vivo Cell Kinetic StudiesCell Kinetic Studies Using a Monoclonal Antibody to Bromodeoxyuridine Immunohistochemical Detection of Cells in the Division Cycle Using Antibodies to Proliferating Cell Nuclear Antigen (PCNA) Double Label Immunohistochemistry on Tissue Sections Using Alkaline Phosphatase and Peroxidase Conjugates Preparation of Colloidal Gold Probes Immunogold Probes in Light Microscopy Immunogold Probes in Electron Microscopy Electron Microscopic-Silver Enhancement for Double Labeling with Antibodies Raised in the Same Species Flow Cytometric Analysis of Cell Surface Antigen Density Multiple Immunofluorescence Analysis of Cells Using Flow CytometryFlow Cytometric Methods of Analyzing Apoptotic Cells Flow Cytometric Detection of Proliferation-Associated Antigens PCNA and Ki-67 Sorting of Human Peripheral Blood T-Cell Subsets Using Immunomagnetic Beads Preparation and Use of Nonradioactive Hybridization Probes Cellular Human and Viral DNA Detection by Nonisotopic In Situ Hybridization Biotinylated Probes in Colony Hybridization Nonisotopic In Situ Hybridization Gene Mapping and Cytogenetics Expression of Recombinant Antibody Fusion Proteins in E. coli Expression of Antibody Fusion Proteins in Mammalian Cells Antibody Screening of Bacteriophage lgt-11 DNA Expression Libraries Display of Antibody Chains on Filamentous Bacteriophage Production of Human Fab Antibody Fragments from Phage Display LibrariesAntibodies from Phage Display Libraries as Immunochemical ReagentsIndex

Review :
a treasury of detailed information, both within the protocols and in the Notes, which in some chapters are extensive will be useful to both those who already have some experience in this area and to those who wish to embark on new techniques. - Microbiology Today