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Home > Mathematics and Science Textbooks > Biology, life sciences > Microbiology (non-medical) > Adenovirus Methods and Protocols: v. 1 Adenoviruses, and Vectors, Quantitation, and Animal Models(v. 130 Methods in Molecular Biology)
Adenovirus Methods and Protocols: v. 1 Adenoviruses, and Vectors, Quantitation, and Animal Models(v. 130 Methods in Molecular Biology)

Adenovirus Methods and Protocols: v. 1 Adenoviruses, and Vectors, Quantitation, and Animal Models(v. 130 Methods in Molecular Biology)


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About the Book

"Adenovirus Methods and Protocols" is designed to help new researchers to conduct studies involving adenoviruses and to help established researchers to branch into new areas. The initial chapters of "Adenovirus Methods and Protocols, Volume 1" address several techniques for construction of adenoviruses for use as vectors and for basic research. The topics include deletion mutants, capsid modifications, insertions, and gene replacements both in human adenoviruses and murine, bovine, and ovine adenoviruses.

Table of Contents:
TABLE OF CONTENTS; Vol. I Topics: Adenoviruses and vectors, quantitation, and animal models; 1. Manipulation of Early Region 4; Julie Boyer and Gary Ketner; 2. Isolation, Growth, and Purification of Defective Adenovirus Deletion Mutants; Gary Ketner and Julie Boyer; 3. Construction of Adenovirus Type 5 Early Region 1 (E1) and 4 (E4) Virus Mutants; Peter Groitl and Thomas Dobner; 4. Construction of Mouse Adenovirus Type 1 Mutants; Angela N. Cauthen, Amanda R. Welton, and Katherine R. Spindler; 5. Generation of Recombinant Adenovirus using E.coli BJ5183 Recombination System; P. Seshidhar Reddy, Shanthi Ganesh, Lynda Hawkins, Neeraja Idamakanti; 6. Production and Release Testing of Ovine AtadenovirusVectors; Gerald W. Both, Fiona Cameron, Anne Collins, Linda J. Lockett, Jan Shaw; 7. Construction of Capsid Modified Recombinant Bovine Adenovirus Type 3; Alexander N. Zakhartchouk, Qiaohua Wu, Suresh K. Tikoo; 8. Adenovirus Capsid Chimeras: Fiber Terminal Exon Insertions / Gene Replacements in the MLTU; Jason Gall, John Schoggins, and Erik Falck-Pedersen; 9. Temperature-Sensitive Replication-Competent Adenovirus shRNA Vectors to Study Cellular Genes in Virus-Induced Apoptosis; T. Subramanian and G. Chinnadurai; 10. Evaluating Apoptosis in Tumor Cells Infected with Adenovirus Expressing p53 Tumor Suppressor Gene; Elizabeth Perron, M. Behzad Zafar, Amanda Pister, Zhen-Guo Wang, Jun Tian and Prem Seth; 11. Growth and Purification of Enteric Adenovirus Type 40; Vivien Mautner; 12. Immunocompetent, Semi-permissive Cotton Rat Tumor Model for the Evaluation of Oncolytic Adenoviruses; Karoly Toth, Jacqueline F. Spencer, William S.M. Wold; 13. The Use of the Syrian Hamster as an Animal Model for Oncolytic Adenovirus Vectors; Maria A. Thomas, Jacqueline F. Spencer, and William S. M. Wold; 14. A Real-Time PCR Method to Rapidly Titer Adenovirus Stocks; Maria A. Thomas, Drew L. Lichtenstein, Peter Krajcsi, and William S. M. Wold; 15. Detection and Quantitation of Subgroup C Adenovirus DNA in Human Tissue Samples by Real-Time PCR; C.T. Garnett, Ching-I Pao, Linda R. Gooding; 16. Flow Cytometric Detection of Adenoviruses and Intracellular Adenovirus Proteins; Graham Bottley, John R. Holt, Nicola J. James and G. Eric Blair; 17. Capture ELISA Quantitation of Mouse Adenovirus Type 1 in Infected Organs; Amanda R. Welton and Katherine R. Spindler; 18. Preparation and Titration of CsCI-Banded Adenovirus Stocks.


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Product Details
  • ISBN-13: 9781597451666
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Edition: Revised edition
  • Sub Title: v. 1 Adenoviruses, and Vectors, Quantitation, and Animal Models
  • ISBN-10: 1597451665
  • Publisher Date: 01 Jan 2007
  • Binding: Digital (delivered electronically)
  • Series Title: v. 130 Methods in Molecular Biology


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Adenovirus Methods and Protocols: v. 1 Adenoviruses, and Vectors, Quantitation, and Animal Models(v. 130 Methods in Molecular Biology)
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