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Home > Mathematics and Science Textbooks > Biology, life sciences > Cellular biology (cytology) > Cell Cycle Protocols: (No. 296 Methods in Molecular Biology)
Cell Cycle Protocols: (No. 296 Methods in Molecular Biology)

Cell Cycle Protocols: (No. 296 Methods in Molecular Biology)


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About the Book

The importance of understanding the fundamental mechanisms that modulate cell division has been reiterated by relatively recent discoveries of links between cell cycle control and DNA repair, growth, cellular metabolism, development and cell death. The aim of Cell Cycle Protocols is to provide a comprehensive set of protocols for studying the eukaryotic cell cycle and its key regulatory molecules, together with overviews of cell cycle regulatory molecules and mechanisms in well-studied organisms.

Table of Contents:
Part I. Overviews of Cell Cycle Control in Different Organisms; 1.Cell cycle molecules and mechanisms of the budding and fission yeasts; Tim Humphrey and Amanda Pearce; 2. The plant cell cycle: an overview; John H. Doonan; 3. The C. elegans cell cycle: Overview of molecules and mechanisms; Sander van den Heuvel; 4. Developmental control of growth and cell cycle progression in Drosophila; Lisa Swanhart, Jeremy Kupsco, and Robert J. Duronio; 5. The Xenopus cell cycle: an overview; Anna Philpott and P. Renee Yew; 6. The mammalian cell cycle: An Overivew; Jane Vera Harper and Gavin Brooks; Part II. Cell Cycle Study Methods and Techniques; 7. Synchronization of cell populations in G1/S and G2/M phases of the cell cycle. Jane Vera Harper; 8. Mapping Origins of DNA Replication in Eukaryotes; Susan A. Gerbi; 9. In situ assay for analyzing the chromatin binding of proteins in fission yeast; Stephen E. Kearsey, Lydia Brimage, Mandana Namdar, Emma Ralph, and Xiaowen Yang; 10. Application of magnetic beads to purify cells transiently transfected with plasmids encoding short hairpin RNAs; Peter D. Adams. 11.The cell cycle and virus infection; Steven Emmett, Brian Dove, Torsten Wurm, and Julian A. Hiscox; 12. Methods for preparation of proteins and protein complexes that regulate the eukaryotic cell cycle for structural studies; Julie Welburn and Jane Endicott; Part III. Detection and Measurement of Cell Cycle Molecules; 13. E2F transcription factors and pRb pocket proteins in cell cycle progression. Ludger Hauck and Rudiger von Harsdorf; 14. Forkhead (FOX) transcription factors and the cell cycle: Measurement of DNA binding by FoxO and FoxM transcription factors; Katrina A. Bicknell; 15. Measurement of Geminin Activity in Xenopus Egg Extracts; Thomas J. McGarry; 16. CDK-activating kinases: detection and activity measurements; Stephane Larochelle and Robert P. Fisher; 17. Cyclins, cyclin-dependent kinases, and cyclin-dependent kinase inhibitors: detection methods and activity measurements; Gavin Brooks; 18. Measurement of Wee Kinase Activity; Paul R. Mueller and Walter F. Leise III; 19. CDC25 dual-specificity protein phosphatases: detection and activity measurements; Sorab N. Dalal and Melanie Volkening; 20. Assaying Cell Cycle Checkpoints: Activity of the Protein Kinase Chk1; Carmela Palermo and Nancy C. Walworth; 21. Polo-like kinase-1: Activity measurement and RNAi-mediated knockdown; Marcel A. T. M. van Vugt and Rene H. Medema; 22. The IpI1/Aurora Kinase Family: Methods of Inhibition and Functional Analysis in Mammalian Cells; Claire Ditchfield, Nicholas Keen, and Stephen S. Taylor; 23. Purification of the Nde80 kinetochore subcomplex from Xenopus eggs; Mark L. McCleland and P. Todd Stukenberg; About the Editors


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Product Details
  • ISBN-13: 9781592598571
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Language: English
  • ISBN-10: 1592598579
  • Publisher Date: 01 Jan 2005
  • Binding: Digital (delivered electronically)
  • Series Title: No. 296 Methods in Molecular Biology


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