Bacterial Artificial Chromosomes
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Home > Mathematics and Science Textbooks > Biology, life sciences > Life sciences: general issues > Genetics (non-medical) > Bacterial Artificial Chromosomes: v.2 Functional Studies
Bacterial Artificial Chromosomes: v.2 Functional Studies

Bacterial Artificial Chromosomes: v.2 Functional Studies


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About the Book

Bacterial Artificial Chromosomes provides a comprehensive collection of the protocols and resources developed for BACs in recent years. These two volumes collectively cover four topics about BACs: 1) library construction, 2) physical mapping, 3) sequencing, and 4) functional studies. The laboratory protocols follow the successful Methods in Molecular Biology series format by containing a clear sequences of steps followed by extensive troubleshooting notes. The protocols cover simple techniques such as BAC DNA purification to complex procedures such as BAC transgenic mouse generation. Both routine and novel methodologies are presented. In addition to protocols, chapter topics include scientific reviews, software tools, database resources, genome sequencing strategies and case studies. These books will provide useful protocols and resources to a wide variety of researchers, including genome sequencers, geneticists, molecular biologists, and biochemists studying the structure and function of the genomes or specific genes.

Table of Contents:
1 Use of BAC End Sequences for SNP Discovery Michael M. Weil, Rashmi Pershad, Ruoping Wang, and Sheng Zhao; 2 Exon Trapping for Positional Cloning and Fingerprinting Scott E. Wenderfer and John J. Monaco; 3 Isolation of CpG Islands from BAC Clones Using a Methyl-CpG Binding Column Sally H. Cross; 4 BAC Microarray-Based Comparative Genomic Hybridization Antoine M. Snijders, Richard Segraves, Stephanie Blackwood, Daniel Pinkel, and Donna G. Albertson; 5 Large DNA Transformation in Plants Sangdun Choi; 6 Retrofitting BACs with A Selectable Marker for Transfection, Zunde Wang, Angelika Longacre, and Peter Engler; 7 BAC Modification Using a RecA Expressing Shuttle Vector System, Nathan Mise and Philip Avner; 8 Bacterial Artificial Chromosome Engineering, Srividya Swaminathan and Shyam K. Sharan; 9 ET Recombination: DNA Engineering Using Homologous Recombination in E. coli, Joep P.P. Muyrers, Youming Zhang, Vladmir Benes, Giuseppe Testa, Jeanette M.J. Rientjes, and A. Francis Stewart; 10 BAC engineering for the generation of ES cell targeting constructs and mouse transgenes, Giuseppe Testa, Kristina Vintersten, Youming Zhang, Vladmir Benes, Joep P.P. Muyrers, and A. Francis Stewart; 11 Microinjection of BAC DNA into The pronucei of Fertilised Mouse Oocytes, Kristina Vintersten, Giuseppe Testa, and A. Francis Stewart; 12 Generation of BAC Transgenic Mice, Vikki M. Marshall, Jan Allison, Tanya Templeton and Simon J. Foote; 13 BAC Rescue: A Tool for Functional Analysis of The Mouse Genome, Deborah A. Swing and Shyam K. Sharan; 14 Herpesviruses: A Brief Overview, Mathias Ackermann; 15 Cloning of [beta]-Herpesvirus Genomes as Bacterial Artificial Chromosomes Eva-Maria Borst, Irena Crnkovic-Mertens, and Martin Messerle; 16 Construction of HSV-1 BACs and Their Use for Packaging of HSV-1 Based Amplicon Vectors Thomas G. H. Heister, A. Vogtlin, Lars Muller, Irma Heid, and Cornel Fraefel; 17 Mutagenesis of viral BACs with linear PCR fragments (ET Recombination) Markus Wagner and Ulrich H. Koszinowski; 18 Mutagenesis of Herpesvirus BAC Plasmids by Allele Replacement Eva-Maria Borst, Gyorgy Posfai, Frank Pogoda, and Martin Messerle; 19 Herpesvirus Genome Mutagenesis by Transposon-Mediated Strategies Alistair McGregor and Mark R. Schleiss; 20 Protective DNA Vaccination by Particle Bombardment Using BAC DNA Containing a Replication-Competent, Packaging-Defective Genome of Herpes Simplex Virus Type I, Mark Suter and Hans Peter Hefti


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Product Details
  • ISBN-13: 9781592597536
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Sub Title: v.2 Functional Studies
  • ISBN-10: 159259753X
  • Publisher Date: /03/2004
  • Binding: Digital online


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