Bacterial Artificial Chromosomes
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Home > Mathematics and Science Textbooks > Biology, life sciences > Microbiology (non-medical) > Bacterial Artificial Chromosomes: v.1 Library Construction,Physical Mapping,and Sequencing
Bacterial Artificial Chromosomes: v.1 Library Construction,Physical Mapping,and Sequencing

Bacterial Artificial Chromosomes: v.1 Library Construction,Physical Mapping,and Sequencing


     0     
5
4
3
2
1



Out of Stock


Notify me when this book is in stock
X
About the Book

Bacterial Artificial Chromosomes provides a comprehensive collection of the protocols and resources developed for BACs in recent years. These two volumes collectively cover four topics about BACs: 1) library construction, 2) physical mapping, 3) sequencing, and 4) functional studies. The laboratory protocols follow the successful Methods in Molecular Biology series format by containing a clear sequence of steps followed by extensive troubleshooting notes. The protocols cover simple techniques such as BAC DNA purification to complex procedures such as BAC transgenic mouse generation. Both routine and novel methodologies are presented. In addition to protocols, chapter topics include scientific reviews, software tools, database resources, genome sequencing strategies and case studies. These books will provide useful protocols and resources to a wide variety of researchers, including genome sequencers, geneticists, molecular biologists, and biochemists studying the structure and function of the genomes or specific genes.

Table of Contents:
1 BAC Library Construction Kazutoyo Osoegawa and Pieter J. de Jong; 2 Construction of Small Genome BAC Library for Functional and Genomic Applications David Smajs, Steven J. Norris, and George M. Weinstock; 3 Preparation of BAC Libraries from Bacterial Genomes by In Vitro Packaging Sangita Pal, Solida Mak, and George M. Weinstock; 4 Exploring TAR Cloning for Selective Isolation of Genomic Regions Natalay Kouprina, Vladimir N. Noskov, Maxim Koriabine, Sun-Hee Leem, and Vladimir Larionov; 5 BAC DNA Purification Tim Svenstrup Poulsen; 6 Hybridization-Based Selection of BAC Clones Chang-Su Lim and Ung-Jin Kim; 7 Applications of Interspersed Repeat Sequence (IRS)-PCR Heike Zimdahl, Claudia Gosele, Thomas Kreitler, and Margit Knoblauch; 8 BAC Mapping by Using Fluorescence In situ Hybridization Xiao-Ning Chen and Julie R. Korenberg; 9 High Throughput BAC Fingerprinting Jacqueline Schein, Tamara Kucaba, Mandeep Sekhon, Duane Smailus, Robert Waterston, and Marco Marra; 10 BAC End Sequencing Tim Svenstrup Poulsen and Hans Erik Johnsen; 11 Radiation Hybrid Mapping with BAC Ends Michael Olivier, Shannon Brady, and David R. Cox; 12 Shotgun Library Construction for DNA Sequencing Bruce A. Roe; 13 Rolling Circle Amplification for Sequencing Templates Paul F. Predki, Chris Elkin, Hitesh Kapur, Jamie Jett, Susan Lucas, Tijana Glavina, and Trevor Hawkins; 14 Transposon-Mediated Sequencing Rachel Dickhoff and Anup Madan; 15 Pyrosequencing: A Tool for DNA Sequence Analysis Elahe Elahi and Mostafa Ronaghi; 16 The Use of Fimers to Eliminate PCR, Primer-Dimer Artifacts and to Increase the Yield in BAC Sequencing Reactions Andrei Malykh, Nikolai Polushin, Alexei Slesarev, and Sergei Kozyavkin; 17 Retrofitting BACs and PACs with LoxP Transposons to Generate Nested Deletions Pradeep K. Chatterjee; 18 Preparing Nested-Deletion Template DNA for FIGE Analyses and Position-Specific End Sequencing with Transposon Primers Pradeep K. Chatterjee and Joseph C. Baker, Jr.; 19 BAC Finishing Strategies Christine Bird and Darren Grafham; 20 Using the TIGR Assembler in Shotgun Sequencing Projects Mihai Pop and Dan Kosack; 21 Finishing "Working Draft" BAC Projects by Directed Sequencing with ThermoFidelase and Fimers Andrei Malykh, Olga Malykh, Nikolai Polushin, Sergei Kozyavkin, and Alexei Slesarev; 22 Optimized Multiplex PCR: an effective method for rapid gap closure Diana Radune and Herve Tettelin; 23 Assembly of DNA Sequencing Data Jeremy Schmutz, Jane Grimwood, and Richard M. Myers; 24 Sequence Finishing Jeremy Schmutz, Jane Grimwood, and Richard M. Myers; 25 Quality assessment of finished BAC sequences Jeremy Schmutz, Jane Grimwood, and Richard M. Myers


Best Sellers


Product Details
  • ISBN-13: 9781592597529
  • Binding: Digital online
  • Sub Title: v.1 Library Construction,Physical Mapping,and Sequencing
  • ISBN-10: 1592597521
  • Language: English


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
Bacterial Artificial Chromosomes: v.1 Library Construction,Physical Mapping,and Sequencing
-
Bacterial Artificial Chromosomes: v.1 Library Construction,Physical Mapping,and Sequencing
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

Bacterial Artificial Chromosomes: v.1 Library Construction,Physical Mapping,and Sequencing

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept

    Fresh on the Shelf


    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!