Germ Cell Protocols
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Germ Cell Protocols: v.1 Sperm and Oocyte Analysis

Germ Cell Protocols: v.1 Sperm and Oocyte Analysis


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About the Book

A gold-standard collection of readily reproducible techniques for the molecular and genetic analysis of germ cells in a variety of different reproductive systems. Volume 1: Sperm and Oocyte Analysis focuses on sperm cells, oocyte analysis, oocyte maturation, fertilization, and preparation techniques. Highlights include in vitro maturation and fertilization of human, porcine, and canine oocytes; the cryopreservation of sperm cells; establishment of an in vitro spermatogenesis system; visualization of sperm accessory structures; and motility assays of stallion spermatozoa. Each readily reproducible protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. For both volumes:

Table of Contents:
Table of Contents; Germ Cell Protocols, Volume 1: Sperm and Oocyte Analysis; Edited by Heide Schatten; 1 Cryopreservation of semen of the Salmonidae with special reference to large scale fertilization Franz Lahnsteiner; 2 Fertilization and sperm chemotaxis in ascidians Manabu Yoshida; 3 Assays for Vertebrate Sperm Chemotaxis" Hitoshi Sugiyama, Bader Al-Anzi, Robert W. McGaughey, and Douglas E. Chandler; 4 Stallion spermatozoa viability: Comparison of Flow Cytometry with Other Methods Katrina Merkies and Mary M. Buhr; 5 Visualization of sperm accessory structures in the mammalian spermatids, spermatozoa and zygotes by immunofluorescence, confocal and immuno-electron microscopy Peter Sutovsky; 6 Localization of Zona Pellucida Receptors on Live Sperm by Fluorophore-Conjugated Solubilized Zona Pellucida Proteins Heather R. Burkin, Ana Paula Alves-Vieira, and David J. Miller; 7 Detection of Human Papillomavirus DNA in Sperm Using the Polymerase Chain Reaction Olufemi A. Olatunbosun, Allison M. Case, and Harry G. Deneer; 8 Spermatogonial stem cells Karim Nayernia, Manyu Li, and Wolfgang Engel; 9 Molecular and cellular approaches for establishment of an in vitro spermatogenesis system Karim Nayernia, Manyu Li, and Wolfgang Engel; 10 Acrosin Activity-Assay for the Evaluation of Mammalian Sperm Acrosome Reaction Yehudit Lax, Sara Rubinstein, and Haim Breitbart; 11 Isolation of sea urchin sperm plasma membranes Kathryn J. Mengerink and Victor D. Vacquier; 12 Detection of centrosome structure in fertilized and artificially activated sea urchin eggs using immunofluorescence microscopy and isolation of centrosomes followed by structural characterization with field emission scanning electron microscopy Heide Schatten and Amitabha Chakrabarti; 13 Microscopic techniques for studying sperm-oocyte interaction during fertilization and early embryo development Mark W. Tengowski; 14 Immunocytochemical Staining of the Metaphase II Spindle in Mammalian Oocytes Steve Mullen and John K. Critser; 15 Spindle Imaging in Living Mammalian Oocytes Using the Liquid-Crystal Polarized Light Microscope and Its Practical Use Wei-Hua Wang, Jimmy Gill, and Cathy Boutin; 16 In Vitro Maturation and Fertilization of Pig Oocytes Heng-Yu Fan and Qing-Yuan Sun; 17 In vitro Maturation and In Vitro Fertilization of Human Oocytes Wei-Hua Wang and Jimmy Gill; 18 In vitro maturation and fertilization of canine oocytes Karine Reynaud, Marie Saint-Dizier, and Sylvie Chastant-Maillard; 19 Detection and Measurement of Membrane Bound Tyrosine Kinases in the Zebrafish Egg Wenjun Wu and William H. Kinsey; 20 A single cell method for estimation of protein tyrosine kinases in the zebrafish egg Elinor MacGregor and William H. Kinsey; 21 Activity of MAPK/p90rsk during Fertilization Qing-Yuan Sun and Heng-Yu Fan


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Product Details
  • ISBN-13: 9781592597444
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Language: English
  • ISBN-10: 1592597440
  • Publisher Date: 14 May 2014
  • Binding: Digital online
  • Sub Title: v.1 Sperm and Oocyte Analysis


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