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Home > Mathematics and Science Textbooks > Biology, life sciences > Cellular biology (cytology) > Germ Cell Protocols: v.2 Molecular Embryo Analysis,Live Imaging,Transgenesis,and Cloning
Germ Cell Protocols: v.2 Molecular Embryo Analysis,Live Imaging,Transgenesis,and Cloning

Germ Cell Protocols: v.2 Molecular Embryo Analysis,Live Imaging,Transgenesis,and Cloning


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About the Book

For volume 2 alone: A gold-standard collection of readily reproducible techniques for the molecular and genetic analysis of germ cells in a variety of different reproductive systems. Volume 2: Molecular Embryo Analysis, Live Imaging, Transgenesis, and Cloning focuses on molecular egg analysis, live egg imaging, nuclear cloning, oocyte cryopreservation, and nuclear transfer. Highlights include the identification and characterization of Oct-4, germline chromatin silencing by RNAi, cDNA subtraction and cloning in the field of trophoblast/placental development, and selective ablation. Each readily reproducible protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Germ Cell Protocols offers both novice and established researchers a gold-standard collection of hard-to-find methods of high impact research, diverse procedures that are easy-to-follow, well-illustrated, and allow a cross-species transfer of knowledge from lower vertebrates to higher mammalian systems.

Table of Contents:
Identification and Characterization of Oct-4, and Germline-Specific Expression of the Oct-4-Green Fluorescent Protein (GFP) Transgene in Mice Michele Boiani, James Kehler, and Hans R. Scholer; Analysis of Germline Chromatin Silencing by Double Stranded RNA-Mediated Interference (RNAi) in Caenorhabditis elegans Monika A. Jedrusik and Ekkehard Schulze; cDNA Subtraction and Cloning in the Field of Trophoblast/Placental Development Myriam Hemberger; PCR-Based Cloning and Differential Screening of RNAs from Xenopus Primordial Germ Cells: Cloning Uniquely Expressed RNAs from Rare Cells Thiagarajan Venkataraman, Elio Dancausse and Mary Lou King; Differentiating Embryonic Stem Cells into Embryonic Bodies Gisele Hopfl, Max Gassmann, Isabelle Desbaillets; The Derivation of Murine Embryonic Stem and Embryonic Germ Cells by Selective Ablation Edward Gallagher and Jim McWhir; Using Multiphoton Excitation to Explore Murky Depths of Developing Embryos Jayne M. Squirrell and John G. White; Dual-Channel Confocal Ratioing of Calcium Dynamics in Living Eggs and Oocytes Steven A. Stricker; A Method for Producing Cloned Pigs by Using Somatic Cells as Donors Liangxue Lai and Randall S. Prather; Pronuclear Microinjection of Mouse Oocytes Thomas Rulicke; Parthenogenesis and Nuclear Transfer in Rabbit Oocytes Yukio Tsunoda and Yoko Kato; Nuclear Transfer in Cattle Gottfried Brem and Manzoor A. Nowshari; In Vitro Fertilization and Embryo Transfer in Felids Charles Earle Pope; Production of Chimeric Chickens Mitsuru Naito and Takashi Kuwana; Production of Haploid and Diploid Androgenetic Bruce P. Brandhorst and Graham E. Corley-Smith; Construction and Detection of Fluorescent, Germ-Line Transgenic Zebrafish Elwood Linney and Ava Udvadia; Production of Zebrafish Germ-Line Chimeras From Cultured Cells Lianchun Fan, Annette Alestrom, Peter Alestrom, and Paul Collodi; Studying Nuclear Reprogramming with Cell Hybrids Steve Pells and Jim McWhir; Cytoplasmic Transfer in Mammalian Eggs and Embryos Henry E. Malter; Conventional Technologies for Cryopreservation of Human Oocytes and Embryos Michael J. Tucker, Sharon Shipley, and Juergen Liebermann; Vitrifying and Warming of Human Oocytes, Embryos and Blastocysts Juergen Liebermann, and Michael J. Tucker; Microfluidic Removal of Cumulus Cells from Mammalian Zygotes David J. Beebe and Henry C. Zeringue; Mammalian Embryo Culture in a Microfluidic Device Eric M. Walters, Sherrie G. Clark, David J. Beebe, and Matthew B. Wheeler; Application of Alginate Gels to the Study of Mammalian Development R. L. Gardner; Using TUNEL in Combination with an Active Caspase-3 Immunoassay to Identify Cells Undergoing Apoptosis in Preimplantation Mammalian Embryos Steve Mullen and John K. Critser


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Product Details
  • ISBN-13: 9781592597413
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Language: English
  • ISBN-10: 1592597416
  • Publisher Date: 14 May 2014
  • Binding: Digital online
  • Sub Title: v.2 Molecular Embryo Analysis,Live Imaging,Transgenesis,and Cloning


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Germ Cell Protocols: v.2 Molecular Embryo Analysis,Live Imaging,Transgenesis,and Cloning
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