HIV Protocols
HIV Protocols: Second Edition(485 Methods in Molecular Biology)

HIV Protocols: Second Edition(485 Methods in Molecular Biology)


     0     
5
4
3
2
1



Available


X
About the Book

Despite major advances in HIV treatment, many areas require more study, in order to create efficacious, potent antiretrovirals that can suppress viral load completely and durably without toxic side effects, to define unknown drug targets and fine-tune known targets, and to better understand the interplay between viral and host factors. In "HIV Protocols, Second Edition", expert researchers provide clear, state-of-the-art methods for the study of HIV. Directed toward three specific goals, this text aims to document up-to-date protocols for select aspects of HIV biology, to bring together both virological and immunological approaches in a single, convenient volume, and to present a comprehensive account of a range of techniques not available in any existing HIV protocol book. As a volume in the highly successful Methods in Molecular Biology™ series, the chapters include brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections containing priceless tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, "HIV Protocols, Second Edition" is an ideal guide to the wide array of techniques used in fundamental or applied research into the biology and pathogenesis of HIV-1.

Table of Contents:
Preparation of Virus Particles and their Analysis.- Methods for Viral RNA Isolation and PCR Amplification for Sequencing of Near Full-Length HIV-1 Genomes.- Purification of HIV-1 Virions by Subtilisin Digestion or CD45 Immunoaffinity Depletion for Biochemical Studies.- Calculating HIV-1 Infectious Titre Using a Virtual TCID50 Method.- Methods To Study Hiv-1 Replication.- Cell-Free Assays for HIV-1 Uncoating.- Real-Time PCR Analysis of HIV-1 Replication Post-entry Events.- Analysis of 2-LTR Circle Junctions of Viral DNA in Infected Cells.- HIV-1 Recombination: An Experimental Assay and a Phylogenetic Approach.- Methods of Preparation and Analysis of Intracellular Reverse Transcription Complexes.- Analysis of Viral and Cellular Proteins in HIV-1 Reverse Transcription Complexes by Co-immunoprecipitation.- Isolation and Analysis of HIV-1 Preintegration Complexes.- Bisarsenical Labeling of HIV-1 for Real-Time Fluorescence Microscopy.- Methods for the Study of HIV-1 Assembly.- Assembly of Immature HIV-1 Capsids Using a Cell-Free System.- Preparation of Recombinant HIV-1 Gag Protein and Assembly of Virus-Like Particles In Vitro.- Methods for the Analysis of HIV-1 Nucleocapsid Protein Interactions with Oligonucleotides.- Methods for Analysis of Incorporation and Annealing Methods for Analysis of tRNALys in HIV-1.- Specialized Approaches To Study Hiv-1 Biology and Pathogenesis.- Somatic Cell Genetic Analyses to Identify HIV-1 Host Restriction Factors.- Rapid, Controlled and Intensive Lentiviral Vector-Based RNAi.- Reverse Two-Hybrid Screening to Analyze Protein–Protein Interaction of HIV-1 Viral and Cellular Proteins.- Methods to Study Monocyte Migration Induced by HIV-Infected Cells.- Novel Mouse Models for Understanding HIV-1 Pathogenesis.- Immunological Studies Of Hiv.-Mucosal Antibody Responses to HIV.- Isolating Mucosal Lymphocytes from Biopsy Tissue for Cellular Immunology Assays.- Quantifying HIV-1-Specific CD8 + T-Cell Responses Using ELISPOT and Cytokine Flow Cytometry.- Multiparameter Flow Cytometry Monitoring of T Cell Responses.- Measuring HIV Neutralization in a Luciferase Reporter Gene Assay.- Assessing the Antiviral Activity of HIV-1-Specific Cytotoxic T Lymphocytes.- Methods for Quantitating Antigen-Specific T Cell Responses Using Functional Assays in Rhesus Macaques.- Drug Resistant Viruses And Viral Evolution.- Isolation of Drug-Resistant Mutant HIV Variants Using Tissue Culture Drug Selection.- Virus Evolution as a Tool to Study HIV-1 Biology.


Best Sellers


Product Details
  • ISBN-13: 9781588298591
  • Publisher: Humana Press Inc.
  • Binding: Hardback
  • Height: 279 mm
  • No of Pages: 457
  • Series Title: 485 Methods in Molecular Biology
  • Width: 210 mm
  • ISBN-10: 1588298590
  • Publisher Date: 08 Dec 2008
  • Edition: Revised edition
  • Language: English
  • Returnable: Y
  • Sub Title: Second Edition


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
HIV Protocols: Second Edition(485 Methods in Molecular Biology)
Humana Press Inc. -
HIV Protocols: Second Edition(485 Methods in Molecular Biology)
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

HIV Protocols: Second Edition(485 Methods in Molecular Biology)

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept

    New Arrivals


    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!