HIV Protocols
HIV Protocols: (v. 17 Methods in Molecular Biology)

HIV Protocols: (v. 17 Methods in Molecular Biology)


     0     
5
4
3
2
1



Out of Stock


Notify me when this book is in stock
X
About the Book

This collection of cutting-edge techniques is essential for studying the molecular biology, virology, and immunology of the HIV virus. The methods cover a broad range of research interests, including quantitation of viral genomes, HIV promoter function, B-cell epitope mapping, viral coreceptor usage, and measurements of T-cell function. Special emphasis is accorded to studying those viral and host immune responses to infection that will be critical to the design of effective preventive vaccines. HIV Protocols is the first HIV methods book to concentrate equally on virology, molecular biology, and immunology, as well as to incorporate methods on chemokine receptor structure and function.

Table of Contents:
Part I. Virology. Isolation and Expansion of HIV from Cells and Body Fluids by Coculture Quantitative HIV Culture Quantitation of Cell-Free HIV by Reverse Transcriptase Activity Determination of Syncytium-Inducing Phenotype of Primary HIV-1 Isolates Using MT-2 Cells Determination of HIV-1 Chemokine Coreceptor Tropism Using Transduced Human Osteosarcoma (HOS) Cells Use of Luciferase Reporter Viruses for Studying HIV Entry A Cell-Cell Fusion Assay to Monitor HIV-1 Env Interactions with Chemokine Receptors Methods of Culturing HIV-1 from Semen Part II. Molecular Biology. Detection of HIV-1 Nucleic Acids by Southern Blotting Detection of HIV-1 Nucleic Acids by Northern Blotting Rapid Identification of Cloned HIV-1 Fragments PCR Amplification and Cloning of Virtually Full-Length HIV-1 Provirus Quantitation of HIV-1gag DNA and RNA from Single Frozen Cell Pellets Quantitative PCR for HIV-1 Proviral DNA Diagnosis and Direct Automated Sequencing of HIV-1 From Dried Blood Spots (DBS) Collected on Filter Paper Quantification of HIV-1 RNA in Dried Plasma Spots (DPS): A Field Approach to Therapeutic Monitoring Quantitation of HIV-1 RNA in Genital Secretions Detection of Nucleic Acids in Cells and Tissues by In Situ Polymerase Chain Reaction Omar Basgasra Determination of the Promoter Activity of HIV-1 Using the Chloramphenical Acetyltransferase Reporter Gene Assay In Vitro Techniques for Studies of HIV-1 Promoter Activity Detection of Polymorphisms in the HIV-1 Coreceptor CCR5 Using Single-Strand Conformation Polymorphism Sequence-Specific Ppriming as a Rapid Screen for Known Mutations Quantitation of HIV-1 Entry Cofactor Expression Retrovirus-Mediated Grene Transduction of SupT1 Cells Cloning of HIV Single-Strand DNA Binding Protein from Human Lymphocyte Lamda gt11 Expression Library with P32-Oligomers Telomeric Terminal Restriction Fragment (TRF) Part III. Humoral Immunology. Serologic Analysis by Enzyme-Linked Immunosorbent Assay (ELISA) Quantitative Immunoblotting with Fusion Proteins Analysis of Antibody Interactions with HIV-1 Envelope Expressed on the Surface of Acutely Infected H9 Cells Analysis of Antibody-Antigen Interactions Using Surface Plasmon Resonance Measurement of HIV-1 Specific and Total Antibody Secreting Cells by ELISPOT Linear Epitope Mapping by the PEPSCAN Method Neutralization of HIV-1 Infection of Human Peripheral Blood Mononuclear Cells (PBMC): Antibody Dilution Method Neutralization of HIV-1 Infection of Human Peripheral Blood Mononuclear Cells (PBMC): Infectivity Reduction Method Collection and Processing of Mucosal Secretions from HIV-1 Infected Women Collection and Processing of Mucosal Secretions from Mice Part IV. Cellular Immunology. Lymphocyte Proliferation Assay HIV-1 Specific Cytotoxic T-Cell Assays HIV-1- Specific Antibody-Dependent Cellular Cytotoxicity (ADCC) Evaluation of Natural Killer Cell Activity Generation and Expansion of HIV-1 CD4+ Antigen-Specific T Cell Lines Immunophenotyping and Assessment of Cell Function by Three-Color Flow Cytometry of Peripheral Blood Lymphocytes Detection of Apoptosis in HIV-Infected Cell Populations using TUNEL Index.

Review :
"... a great companion to the more general laboratory protocol manuals... provide[s] an impressively up-to-date collection of laboratory methods for every common and not-so-common AIDS lab experiment." - Antiviral Research HIV Protocols is a good addition to a basic science laboratory that processes various HIV clinical specimens. Graduate students or clinical fellows starting out on their careers in basic experimental science will find it most useful. Overall, this book compiles a number of general basic science protocols in the fields of virology, molecular biology and immunology as applied to the study of HIV. The thorough nature of the materials and methods section of each chapter ensures that the reader will get off to a good start when using these techniques in their own laboratory. - Can J Infect Dis


Best Sellers


Product Details
  • ISBN-13: 9780896033696
  • Publisher: Humana Press Inc.
  • Binding: Hardback
  • Language: English
  • Series Title: v. 17 Methods in Molecular Biology
  • Width: 229 mm
  • ISBN-10: 0896033694
  • Publisher Date: 09 Feb 1999
  • Height: 152 mm
  • Returnable: N
  • Weight: 908 gr


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
HIV Protocols: (v. 17 Methods in Molecular Biology)
Humana Press Inc. -
HIV Protocols: (v. 17 Methods in Molecular Biology)
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

HIV Protocols: (v. 17 Methods in Molecular Biology)

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept

    New Arrivals


    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!