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Home > Mathematics and Science Textbooks > Biology, life sciences > Microbiology (non-medical) > Calcium Signaling Protocols: (312 Methods in Molecular Biology)
Calcium Signaling Protocols: (312 Methods in Molecular Biology)

Calcium Signaling Protocols: (312 Methods in Molecular Biology)


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About the Book

Calcium Signaling Protocols: Second Edition provides an important update to the acclaimed first edition with new and cutting-edge methods in this important field. Sections cover specialist measurement systems, measurement of Ca2+ channel activity, measurement of Ins(1,4,5)P3 and Ca2+ release, specialist measurement techniques and Ca2+ sensitive targets.

Table of Contents:
General.- Fluorescent Measurement of [Ca2+]c.- Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2.- Measurement of [Ca2+]i in Cell Suspensions Using Indo-1.- Specialist Measurement Systems.- Confocal Microscopy.- Single-Cell and Subcellular Measurement of Intracellular Ca2+ Concentration.- Ratiometric Ca2+ Measurements Using the FlexStation® Scanning Fluorometer.- Measuring Ca2+ Changes in Multiwell Format Using the Fluorometric Imaging Plate Reader.- Measurement of Ca2+ Channel Activity.- Measurement of Ca2+ Entry Using 45Ca2+.- Measurement of [3H]PN200-110 and [125I]?-Conotoxin MVIIA Binding.- Whole-Cell Patch Clamp Recording of Voltage-Sensitive Ca2+ Channel Currents.- Measurement of Ins(1,4,5,)P3 and Ca2+ Release From Intracellular Stores.- Measurement of Phospholipase C by Monitoring Inositol Phosphates Using [3H]Inositol-Labeling Protocols in Permeabilized Cells.- Measurement of Inositol(1,4,5)Trisphosphate Using a Stereospecific Radioreceptor Mass Assay.- Measurement of Calcium Fluxes in Permeabilized Cells Using a 45Ca2+ Uptake and Release Assay.- Microinjection of myo-Inositol(1,4,5)Trisphosphate and Other Calcium-Mobilizing Agents Into Intact Adherent Cells.- Measurement of Free [Ca2+] Changes in Agonist-Sensitive Internal Stores Using Compartmentalized Fluorescent Indicators.- Specialist Measurement Techniques.- Measurement of [Ca2+]i in Smooth Muscle Strips Using Front-Surface Fluorimetry.- Measurement of Calcium and Movement in Heart Cells.- Simultaneous Analysis of Intracellular pH and Ca2+ From Cell Populations.- Measurement of Cytosolic-Free Ca2+ in Plant Tissue.- Ca2+-Sensitive Targets.- Assay and Purification of Calmodulin-Dependent Protein Kinase.- Assay and Purification of Calmodulin-Dependent Cyclic Nucleotide Phosphodiesterase andIsozyme Separation.- Measurement of Ca2+-ATPase Activity (in PMCA and SERCA1).

Review :
From Reviews of the First Edition... "...will find a place on the bench in any lab that is moving into calcium signaling, or expanding its repertoire of techniques in calcium signaling. It will be valuable as a resource for...years to come." -Cell Biology International "This book achieves much...For anyone remotely interested in Ca2+ it will be a very valuable resource in their lab" -Quarterly Review of Biology ". . .recommended to investigators interested in calcium measurement using optical methods. "-Doody's Health Sciences Book Review Journal "Most protocols identify required materials such as buffers, reagents and cell lines. Methods are presented in a step-by-step format. Hopefully these protocols are adaptable by scientists for use in specific research. Certainly, the methods are specific enough not to leave the researcher stranded. Calcium Signaling Protocols is recommended for academic health and science libraries who support academic research."-E-Streams


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Product Details
  • ISBN-13: 9781588294425
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Edition: Revised edition
  • Language: English
  • Returnable: Y
  • Width: 155 mm
  • ISBN-10: 1588294420
  • Publisher Date: 15 Aug 2005
  • Binding: Hardback
  • Height: 235 mm
  • No of Pages: 359
  • Series Title: 312 Methods in Molecular Biology


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