About the Book
David Lambert and a panel of leading authorities present a wide range of experimental protocols for studying Ca2+ signaling. These optimized techniques cover the more common applications, including 45Ca2+ flux measurements, and basic fluorometric technology, as well as more sophisticated methods, including confocal microscopy and subcellular Ca2+ imaging. There are also methods - largely based on fluorescence measurement - to determine Ca2+ channel activity and the release of Ca2+ from intracellular stores. Calcium Signaling Protocols offers today's researchers readily reproducible laboratory methods that make it possible to examine the calcium signaling process in detail in a range of cells of animal and plant origin.
Table of Contents:
Part I. General. Fluorescent Measurement of [Ca2+]c: Basic Practical Considerations, Alec W. M. Simpson. Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2, Robert A. Hirst, Charlotte Harrison, Kazuyoshi Hirota, and David G. Lambert. Measurement of [Ca2+]i in Cell Suspensions Using Indo-1, Adriaan Nelemans. Part II. Specialist Measurement Systems. Confocal Microscopy: Theory and Applications, Laura C. Mongan, Joanne Gormally, Andrew R. D. Hubbard, Christopher d'Lacey, and Colin D. Ockleford. Measurement of Intracellular Calcium Concentration Using Confocal Microscopy, Carmen Perez-Terzic, Marisa Jaconi, and Lisa Stehno-Bittel. Single Cell and Subcellular Measurement of Intracellular Ca2+ Concentration ([Ca2+]i), Anthony J. Morgan and Andrew P. Thomas. Measurement of [Ca2+] Using the Fluorometric Imaging Plate Reader (FLIPR), Elaine Sullivan, Emily M. Tucker, and Ian L. Dale. Part III. Nonelectrophysiological Measurement of Ca2+ Channel Activity. Measurement of Ca2+ Entry Using 45Ca2+, Mercedes Villarroya, Manuela G. Lopez, Maria F. Cano-Abad, and Antonio G. Garcia. Measurement of [3H]PN200-110 and [125I]w-Contoxin MVIIA Binding, Kazuyoshi Hirota and David G. Lambert. Part IV. Measurement of Ins(1,4,5)P3 and Ca2+ Release From Intracellular Stores. Measurement of Inositol (Poly)phosphate Formation Using [3H]Inositol Labeling Protocols in Permeabilized Cells, Philip Swigart and Shamshad Cockcroft. Measurement of Inositol(1,4,5)triphosphate Using a Stereospecific Radioreceptor Mass Assay, Darren Smart. Measurement of Calcium Fluxes in Permeabilized Cells Using a 45Ca2+ Uptake and Release Assay, Robert A. Wilcox. Microinjection of myo-Inositol(1,4,5)triphosphate and Other Calcium-Mobilizing Agents into Intact Adherent Cells, Robert A. Wilcox, Ian D. Forsythe, and Terence J. McCann. Photolysis of Caged Calcium Using a Low-Cost Flash Unit, Gommert A. van Koeveringe and Ron van Mastrigt. Measurement of Ca2+ Flux Through Ins(1,4,5)P3 Receptor - Ca2+ Channels in Lipid Bilayers ("Dip-Tip" and "Schindler" Methodology), Edwin C. Thrower. Continuous Fluorescent Monitoring of Cellular Calcium Fluxes: A Novel Perfusion System for the Investigation of Inosito(1,4,5)trisphosphate-Dependent Quantal Calcium Release Using Immobilized, Electropermeabilized Cells, Robert A. Wilcox and James Strupish. Measurement of Free [Ca2+] Changes in Agonist-Sensitive Internal Stores Using Compartmentalized Fluorescent Indicators, Aldebaran M. Hofer. Part V. Specialist Measurement Techniques. Measurement of [Ca2+]i in Smooth Muscle Strips Using Front-Surface Fluorimetry, Hideo Kanaide. Measurement of Calcium and Movement in Heart Cells, Leong L. Ng and Paulene A. Quinn. Simultaneous Analysis of Intracellular pH and Ca2+ from Cell Populations, Raul Martinez-Zaguilan, Linda S. Tompkins, Robert J. Gillies, and Ronald M. Lynch. Measurement of Cytosolic-Free Ca2+ in Plant Tissue, Martin R. McAinsh and Irina Staxen. Part VI. Ca2+ Sensitive Targets. Assay and Purification of Calmodulin-Dependent Protein Kinase, Rakesh Kakkar and Rajendra K. Sharma. Measurement of Ca2+-ATPase Activity (in PMCA and SERCA1), Danuta Kosk-Kosicka. Index.
Review :
: "...recommended to investigators interested in calcium measurement using optical methods. "-Doody's Health Sciences Book Review Journal "Most protocols identify required materials such as buffers, reagents and cell lines. Methods are presented in a step-by-step format. Hopefully these protocols are adaptable by scientists for use in specific research. Certainly, the methods are specific enough not to leave the researcher stranded. Calcium Signaling Protocols is recommended for academic health and science libraries who support academic research."-E-Streams
