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Home > Medicine & Health Science textbooks > Medical specialties, branches of medicine > Pathology > Medical microbiology and virology > Hepatitis B and D Protocols: Volume 2: Immunology, Model Systems, and Clinical Studies(96 Methods in Molecular Medicine)
Hepatitis B and D Protocols: Volume 2: Immunology, Model Systems, and Clinical Studies(96 Methods in Molecular Medicine)

Hepatitis B and D Protocols: Volume 2: Immunology, Model Systems, and Clinical Studies(96 Methods in Molecular Medicine)


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About the Book

For volume 2: A broad-ranging collection of cutting-edge techniques for the study of HBV and HDV infections and for the development of therapies to treat them. In this second of two volumes, Immunology, Model Systems, and Clinical Studies, the authors focus on protocols for the study of host immune responses to infection, in vitro and in vivo models of infection, and the development of antivirals. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle underlying the technique, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. An accompanying first volume, Detection, Genotypes, and Characterization, contains user-friendly protocols for the identification and quantification of viral markers, the detection and impact of viral variants, and the study of the viral life cycle. For both volumes: A broad-ranging collection of core techniques for the study of HBV and HDV infections and for the development of therapies to treat them. The first volume Detection, Genotypes, and Characterization, the authors focus on readily reproducible molecular methods for the identification and quantification of viral markers, the detection and impact of viral variants, and the study of the viral life cycle. The second volume, Immunology, Model Systems, and Clinical Studies, contains user-friendly protocols for the study of host immune responses to infection, in vitro and in vivo models of infection, and the development of antivirals. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle underlying the technique, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. The set offers both new and experienced investigators an encyclopedic collection of powerful tools for studying HBV and HDV infections, an essential resource for finding new therapies to treat chronically infected patients.

Table of Contents:
Viral-Specific Immunological and Other Host Responses.- Studying Host Immune Responses Against Duck Hepatitis B Virus Infection.- Measurement of Cell-Mediated Immune Response in Woodchucks.- Study of Liver-Specific Expression of Cytokines During Woodchuck Hepatitis Virus Infection.- Induction of Anti-Hepatitis B Virus Immune Responses Through DNA Immunization.- Monitoring Gene Expression Using DNA Microarrays During Hepatitis B Virus Infection.- Determination of Hepatitis B Virus-Specific CD8+ T-Cell Activity in the Liver.- Determining the Precursor Frequency of HBV Nucleocapsid Antigen-Specific T Cells.- Detection and Characterization of Virus-Specific CD8+ T Cells Using the Tetramer Approach.- In Vitro Analysis of Hepatitis B Virus Specific CD4+ T Cells.- Induction of Humoral and Cellular Immune Responses to Hepatitis Delta Virus Through DNA Immunization in BALB/c Mice.- In Vitro and In Vivo Models.- Infection of Primary Chimpanzee Hepatocytes with Recombinant Hepatitis D Virus Particles.- Study of the Endocytosis and Intracellular Localization of Subviral Particles of Hepatitis B Virus in Primary Hepatocytes.- The Tupaia Model for the Study of Hepatitis B Virus.- Delivery of Hepatitis B Virus Therapeutic Agents Using Asialoglycoprotein Receptor-Based Liver-Specific Targeting.- Woodchuck Hepatitis Virus Hepatocyte Culture Models.- Duck Hepatitis B Virus Primary Hepatocyte Culture Model.- Enhancement of Infection of HepG2 Cells in Culture by Predigestion of Hepadnavirus with V8 Protease.- Construction of Recombinant Adenoviruses that Produce Infectious Hepatitis B Virus.- Baculovirus-Mediated Gene Transfer for the Study of Hepatitis B Virus.- Transgenic Hepatitis B Virus Mouse Model in the Study of Chemotherapy.- Transplantation of Human Hepatocytes in ImmunodeficientUPA Mice.- Duck Hepatitis B Virus Model in the Study of Hepatitis B Virus.- Hepatitis B Virus Transgenic Severe Combined Immunodeficient Mouse Model of Acute and Chronic Liver Disease.- The Chimpanzee Model.- Hepatitis B in Liver Transplant Recipients as a Special Model of Antiviral Drug Development.- Hepatitis Delta Virus Transfection for the Mouse In Vivo Model.- Hepatitis Delta Virus RNA Transfection for the Cell Culture Model.- Antiviral Testing and Clinical Studies.- Analysis of Hepatitis B Virus Dynamics and Its Impact on Antiviral Development.- Genotyping Anti-Hepatitis B Virus Drug Resistance.- A Parsimonious Method for Screening Drug Combinations for Antihepadnaviral Activity Using a Parametric Dose-Response Surface Approach.- Hepatitis B Virus.- Designing Studies to Evaluate Anti-Hepatitis B Virus Therapies.- Specific Considerations in the Design of Hepatitis B Virus Clinical Studies in the Far East.- Studying the Treatment of Chronic Hepatitis B Viral Infection in Special Populations.- Designing Clinical Development Programs for Anti-Hepatitis B Virus Drugs.- Testing Antivirals Against Hepatitis Delta Virus.

Review :
"Both volumes provide an excellent reference for background information and detailed experimental investigations for both Hepatitis B and Hepatitis D. A major attribute of the volumes is that as they cover a wide range of subjects, the reader has the opportunity to access information they would not normally encounter. Volumes 1 and 2 are an excellent reference source and the methodologies described present the opportunity for both new and experienced researchers to study the molecular aspects of HBV and HDV infection."-SGM Quarterly "...an excellent reference for background information and detailed experimental investigations for both Hepatitis B and Hepatitis D..." - Microbiology Today


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Product Details
  • ISBN-13: 9781588291080
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Height: 235 mm
  • No of Pages: 572
  • Series Title: 96 Methods in Molecular Medicine
  • Width: 155 mm
  • ISBN-10: 1588291081
  • Publisher Date: 28 Jan 2004
  • Binding: Hardback
  • Language: English
  • Returnable: Y
  • Sub Title: Volume 2: Immunology, Model Systems, and Clinical Studies


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Hepatitis B and D Protocols: Volume 2: Immunology, Model Systems, and Clinical Studies(96 Methods in Molecular Medicine)
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