Basic Techniques in Biochemistry, Microbiology and Molecular Biology
Home > Medicine & Health Science textbooks > Nursing and ancillary services > Biomedical engineering > Basic Techniques in Biochemistry, Microbiology and Molecular Biology: Principles and Techniques(Springer Protocols Handbooks)
Basic Techniques in Biochemistry, Microbiology and Molecular Biology: Principles and Techniques(Springer Protocols Handbooks)

Basic Techniques in Biochemistry, Microbiology and Molecular Biology: Principles and Techniques(Springer Protocols Handbooks)

|
     0     
5
4
3
2
1




Available


About the Book

This book presents key methodologies, tools and databases for biochemistry, microbiology and molecular biology in simple and straightforward language. Covering all aspects related to experimental principles and procedures, the protocols included here are brief and clearly defined, and include essential precautions to be taken while conducting experiments. The book is divided into two major sections: one on constructing, working with, and standard operating procedures for laboratory instruments; and one on practical procedures used in molecular biology, microbiology and biochemical analysis experiments, which are described in full. Each chapter describes both the basic theory and relevant practical details for a given experiment, and helps readers recognize both the experiment’s potential and limitations. Intended as an intensive introduction to the various tools used in molecular biology, the book covers all basic methods and equipment, including cloning, PCR, spectrophotometers, ELISA readers, sonicators, etc. As such, it offers a valuable asset for final year undergraduate (especially project) students, graduate research students, research scientists and technicians who wish to understand and employ new techniques in the field of biotechnology.

Table of Contents:
Chapter 1. BOD (Biochemical oxygen demand or biological oxygen demand) Incubator.- Chapter 2. Laminar Air Flow/ Biosafety Cabinets.- Chapter 3. Aseptic Hood.- Chapter 4. Autoclave.- Chapter 5. Hot Air Oven.- Chapter 6. Deep Freezer (-20 0C) (Low Temperature Cabinet).- Chapter 7. Refrigerator.- Chapter 8. Compound Microscope.- Chapter 9. Digital Colony Counter.- Chapter 10. Digital Turbidity Meter.- Chapter 11. Digital Nephel Ometer.- Chapter 12. Digital Colorimeter.- Chapter 13. Digital UV / Visible Spectrophotometer.- Chapter 14. Polymerase Chain Reaction.- Chapter 15. ELISA Reader.- Chapter 16. Sonicator.- Chapter 17. Enzyme Assay: Qualitative and Quantitative (Protease, Amylase, Penicillinase, Pectinolytic Enzyme, Chitinase, Keratinase).- Chapter 18. Antimicrobial Sensitivity Assay.- Chapter 19. Extraction of DNA from plant cells.- Chapter 20. Extraction of DNAfrom Animal cells.- Chapter 21. Extraction of DNA and RNA from microorganism.- Chapter 22. Estimation of DNA by Diphenylamine Reaction.- Chapter 23. Estimation of RNA using Orcinol Method.- Chapter 24. Determination of Melting Temperature of DNA.- Chapter 25. Quantitative Estimation of DNA AND RNA.- Chapter 26. Purification and Bioassay of Interleukin 1 and 2 (IL 1 AND IL 2).- Chapter 27. Object: Quantitative Determination of TNF Alpha and Interleukin 6 in the given sample.- Chapter 28. Sterilization of Glassware, Preparation of Media and its Sterilization.- Chapter 29. Sub Culturing of Bacteria, Fungi and Actinomycetes.- Chapter 30. Preservation of Microorganisms: Stabs, Slants, Lyophilization, Cryopreservation.- Chapter 31. Staining Methods: Simple, Negative, Gram Staining, Acid-Fast Staining, Cell Wall, Capsule, Flagella, Nuclear material, Cytoplasm, Filamentous Fungi, Mycoplasma.- Chapter 32. Isolation of Coprophilous Fungi (By Moist Chamber Method).- Chapter 33. Isolation of Microorganisms from air.- Chapter 34. Motility Testing: Hanging Drop Method and Stab.- Chapter 35. Sterility Testing of Pharmaceuticals.- Chapter 36. Physical, Chemical and Bacteriological Analysis of Water. (a) Physical and Chemical Analysis of Water (b) Bacteriological Analysis of Water.- Chapter 37. To Perform Biochemical Identification of Microorganism: Imvic, Oxidative Fermentation, Carbohydrate Source Utilization.- Chapter 38. To Perform Identification of Microorganism by Urease Test.- Chapter 39. Immobilization of Enzymes and Microorganisms.- Chapter 40. Qualitative Systematic Analysis of Carbohydrates (Glucose, Fructose, Lactose, Maltose, Sucrose and Starch).- Chapter 41. Protein Analysis in Food.- Chapter 42. Identification Tests for Proteins (Casein and Albumin).- Chapter 43. Quantitative Analysis of Reducing Sugars by 3, 5-Dinitrosalicylic Acid (Dnsa Method).- Chapter 44. Quantitative Analysis of Proteins by Various Methods Including Biuret.- Chapter 45. Qualitative Anaylsis of Urine for Abnormal Constituents.- Chapter 46. Determination of Blood Creatinine.- Chapter 47. Determination of Blood Sugar.- Chapter 48. To Perform Oral Glucose Tolerance Test.- Chapter 49. Determination of Serum Total Cholesterol.- Chapter 50. Preparation of Buffer Solution and Measurement of PH.- Chapter 51. Study of Enzymatic Hydrolysis of Starch.- Chapter 52. Determination of Salivary Amylase Activity.- Chapter 53. Study the Effect of Temperature on Salivary Amylase Activity.- Chapter 54. Study the Effect of Substrate Concentration on Salivary Amylase Activity.- Chapter 55. Analysis of Butter.- Chapter 56. Analysis of Milk.- Chapter 57. Tlc of Carbohydrates.- Chapter 58. Tlc of Amino Acid.- Chapter 59. Paper Chromatography of Amino Acid.- Chapter 60. Paper Chromatography of Carbohydrates.- Chapter 61. Fat Characterization.- Chapter 62. Analysis of Bread.


Best Sellers


Product Details
  • ISBN-13: 9781493998630
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Height: 254 mm
  • No of Pages: 282
  • Series Title: Springer Protocols Handbooks
  • Width: 178 mm
  • ISBN-10: 1493998633
  • Publisher Date: 04 Aug 2021
  • Binding: Paperback
  • Language: English
  • Returnable: Y
  • Sub Title: Principles and Techniques


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
Basic Techniques in Biochemistry, Microbiology and Molecular Biology: Principles and Techniques(Springer Protocols Handbooks)
Humana Press Inc. -
Basic Techniques in Biochemistry, Microbiology and Molecular Biology: Principles and Techniques(Springer Protocols Handbooks)
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

Basic Techniques in Biochemistry, Microbiology and Molecular Biology: Principles and Techniques(Springer Protocols Handbooks)

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept

    New Arrivals

    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!