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Home > Mathematics and Science Textbooks > Biology, life sciences > Zoology and animal sciences > Analysis of Vitellogenin Gene (Mevg2) from the Sand Shrimp (Metapenaeusensis): Gene Organization and Expression Study
Analysis of Vitellogenin Gene (Mevg2) from the Sand Shrimp (Metapenaeusensis): Gene Organization and Expression Study

Analysis of Vitellogenin Gene (Mevg2) from the Sand Shrimp (Metapenaeusensis): Gene Organization and Expression Study


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About the Book

This dissertation, "Analysis of Vitellogenin Gene (MeVg2) From the Sand Shrimp (Metapenaeusensis): Gene Organization and Expression Study" by Sin-yan, Kung, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Abstract of thesis entitled ANALYSIS OF VITELLOGENIN GENE (MeVg2) FROM THE SAND SHRIMP (Metapenaeus ensis): GENE ORGANIZATION AND EXPRESSION STUDY Submitted by Kung Sin Yan for the degree of Master of Philosophy at The University of Hong Kong in August 2004 The major egg yolk protein, vitellogenin (Vg), is the main nutrient that provides energy and amino acids for embryogenesis and early larval development in shrimp. The study of vitellogenin production is of crucial importance biologically and economically. Therefore, the molecular characterization of the gene encoding vitellogenin is of immense importance to a better understanding of the reproduction in shrimp. In this study, a second form of vitellogenin gene (MeVg2) has been cloned and characterized. Like MeVg1, the MeVg2 cDNA is about 8 kb in size. It encodes a precursor of 2,560 amino acid residues with a predicted molecular mass of 285 kDa. Unlike MeVg1, MeVg2 consists of fewer exons. The proximal 5' region of MeVg2 consists of the TATA box and a Sp1 binding site. In addition, half-site of estrogen responsive element (ERE) and putative binding sites for GATA and heat shock factor (HSF) were identified. By a promoter functional assay, removal of the putative HSF binding sites caused a significant increase in promoter activity, suggesting that HSF may negatively regulate vitellogenin gene expression. Northern blot analysis was used to study MeVg2 expression in different reproductive stages. The results revealed the presence of an 8 kb mRNA and smaller transcripts of 2 to 4 kb. These smaller transcripts constitute a high proportion of the total Vg transcripts and could be the products from an un-identified homologous gene or the alternatively spliced products of MeVg2. Since an alternatively spliced transcript of MeVg1 was identified, the smaller transcripts from the Vg gene may be important for the production of vitellogenin in the hepatopancreas. To study the effect of juvenile hormones on Vg gene expression, in vitro explant cultures were performed with hepatopancreas tissue fragments from reproductive shrimp. Preliminary results showed that farnesoic acid, methyl farnesoate and juvenile hormone III stimulated Vg gene expression in stage III and IV shrimps, but had no effect on stage I shrimp. As compared to other hormones, methyl farnesoate had the strongest effect in stimulating MeVg2 expression. In conclusion, molecular characterization and expression studies of MeVg2 were conducted. The results obtained from this study have provided a greater insight to vitellogenesis in shrimp and a framework to study the functions of terpenoid hormones on vitellogenin gene expression. DOI: 10.5353/th_b3049729 Subjects: LipoproteinsGene expressionShrimps - Molecular genetics


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Product Details
  • ISBN-13: 9781374721685
  • Publisher: Open Dissertation Press
  • Publisher Imprint: Open Dissertation Press
  • Height: 279 mm
  • Sub Title: Gene Organization and Expression Study
  • Width: 216 mm
  • ISBN-10: 1374721689
  • Publisher Date: 27 Jan 2017
  • Binding: Paperback
  • Spine Width: 8 mm
  • Weight: 358 gr


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