Effects of 5-Hydroxytryptamine (5ht) Injection on the Hemolymph Glucose Level and Gene Expression of Reproductive Shrimp (Metapenaeus Ensis)
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Home > Mathematics and Science Textbooks > Biology, life sciences > Zoology and animal sciences > Effects of 5-Hydroxytryptamine (5ht) Injection on the Hemolymph Glucose Level and Gene Expression of Reproductive Shrimp (Metapenaeus Ensis)
Effects of 5-Hydroxytryptamine (5ht) Injection on the Hemolymph Glucose Level and Gene Expression of Reproductive Shrimp (Metapenaeus Ensis)

Effects of 5-Hydroxytryptamine (5ht) Injection on the Hemolymph Glucose Level and Gene Expression of Reproductive Shrimp (Metapenaeus Ensis)


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This dissertation, "Effects of 5-hydroxytryptamine (5HT) Injection on the Hemolymph Glucose Level and Gene Expression of Reproductive Shrimp (metapenaeus Ensis)" by Yan-yee, Lam, 林茵儀, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Abstract of thesis entitled EFFECTS OF 5-HYDROXYTRYPTAMINE (5HT) INJECTION ON THE HEMOLYMPH GLUCOSE LEVEL AND GENE EXPRESSION OF REPRODUCTIVE SHRIMP (Metapenaeus ensis) Submitted by Lam Yan Yee for the degree of Master of Philosophy at The University of Hong Kong in August 2003 The neurotransmitter 5HT (serotonin) is known to regulate many physiological and behavioral processes in crustaceans. It appears precociously during development. Accumulated evidence supports the diverse regulatory roles of 5HT in the control of carbohydrate metabolism, growth and reproduction of crustaceans. The diverse regulatory functions of 5HT are believed to be mediated through the modulations of neurohormones from the neuroendocrine centers. However, it is still not clear how 5HT exerts those effects. To study the hyperglycemic effect of 5HT injection in shrimp, the first part of the study aims to investigate the effects of 5HT injections on the hemolymph glucose level and its relationship to the Crustacean Hyperglycemic Hormone (CHH). At a -8 concentration of 1.0X10 M, the highest hyperglycemic effect was recorded at 20 min after 5HT injection (10 l). Moreover, the hyperglycemic effect is much more pronounced in female shrimp. Since transcripts of CHH-B in eyestalk increases and eyestalk ablation decreases this hyperglycemic effect at 20 min, it is possible that 5HT-induced hyperglycemia is mediated partly by increased CHH-B production and secretion in the eyestalk. However, since this hyperglycemic effect still persists in the eyestalkless shrimp, other hyperglycemic factor(s) in sources other than the eyestalk may also be involved in 5HT-induced hyperglycemia. Coincidently, a neuroparsin precursor-like cDNA (Me-NPP) is isolated from the ovary in the shrimp with the CHH primers. Its expression in the eyestalk is also changed after 5HT treatments. The X-organ sinus gland complex (XOSG) in the eyestalk is the major neurosecretory system for the synthesis, storage and release of neurohormones in crustaceans. Because the molecular basis of 5HT in regulating growth and reproduction of the shrimp is poorly understood, the second part of the study aims to investigate the early transcriptional regulations of 5HT in the shrimp. To study the effects of 5HT on early gene regulations in the eyestalk XOSG, differential display and subtractive hybridization methods have been used. Differential display determines the highest response of differential expressions at 40 min after 5HT administrations. Over 193 clones representing genes regulated by 5HT injection are revealed by subtractive hybridization analysis. Thirty-three potential differentially expressed clones are isolated by differential screening. Reverse transcription-polymerase chain reaction (RT-PCR) confirms the up regulations of several genes. These genes include arrestin, farnesoic acid O-methyl transferase (FAMeT), triosephosphate isomerase (TIM) and gamma aminobutyric acid receptor A (GABA ) subunit α5. The results of this study indicate that 5HT may have a broad spectrum of gene regulations as well as the control of neuropeptide secretion. DOI: 10.5353/th_b2778545 Subjects: Serotonin Blood sugar Shrimps - Genetics


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Product Details
  • ISBN-13: 9781374711914
  • Publisher: Open Dissertation Press
  • Publisher Imprint: Open Dissertation Press
  • Height: 279 mm
  • No of Pages: 124
  • Weight: 581 gr
  • ISBN-10: 1374711918
  • Publisher Date: 27 Jan 2017
  • Binding: Hardback
  • Language: English
  • Spine Width: 8 mm
  • Width: 216 mm


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Effects of 5-Hydroxytryptamine (5ht) Injection on the Hemolymph Glucose Level and Gene Expression of Reproductive Shrimp (Metapenaeus Ensis)
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