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Characterization of Hmscs Transmigrated Through Collagen Barrier

Characterization of Hmscs Transmigrated Through Collagen Barrier


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About the Book

This dissertation, "Characterization of HMSCs Transmigrated Through Collagen Barrier" by Yin-kwai, Wong, 王現葵, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Stem cell therapy is a promising approach for tissue regeneration but there exists a problem of low engraftment rate to the injury site. Our laboratory has shown that hMSCs that were capable to penetrate through collagen barrier have higher migration capacity and engraftment efficiency than those remained inside the collagen matrix and those in traditional 2D culture. These cells capable of penetrating through collagen barrier might be hopeful candidate for improving engraftment efficacy. Major processes of engraftment, such as transmigration through basement membrane and invasion to the site of injury, involve cell-extracellular matrix-interacting proteins. As matrix metalloproteinases (MMP) and integrins are the key players in these processes, MMP and integrin profiles of the hMSCs were studied In this study, we demonstrated that hMSCs that were capable of penetrating through the collagen barrier have distinctive MMP profile to traditional 2D culture. These cells secrete significantly higher amount of MMP-1 than 2D culture, but the amount of MMP-2 secreted is comparable to traditional 2D culture. On the other hand, MMP-9 and MMP-13 were below detection limit by ELISA in both groups. Moreover, we have investigated the subcellular localization of MMPs and integrins. The cells were seeded on dishes with or without ECM coatings. It was demonstrated that hMSCs capable of penetrating through collagen barrier exhibit higher amount of subcellular MT1-MMP and integrin 6271 on ECM coated dish. Moreover, these cells exhibit a prominent feature of perinuclear localization of MT1-MMP., whereas the level of subcellular MMP-2, integrin 65 and 6v73 is comparable to that in 2D culture. We have also investigated the stem properties of hMSCs penetrated through collagen barrier. These properties include proliferation capacity, self-renewal capacity and differentiation potential towards chondrogenic, osteogenic and adipogenic lineages. It has been demonstrated that these properties are not compromised for superior migratory activities. DOI: 10.5353/th_b4786972 Subjects: Stem cells Mesenchyme


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Product Details
  • ISBN-13: 9781361275559
  • Publisher: Open Dissertation Press
  • Publisher Imprint: Open Dissertation Press
  • Height: 279 mm
  • No of Pages: 146
  • Weight: 354 gr
  • ISBN-10: 1361275553
  • Publisher Date: 26 Jan 2017
  • Binding: Paperback
  • Language: English
  • Spine Width: 8 mm
  • Width: 216 mm


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