The X-Encoded Nucleosome Assembly Protein Tspyl2 Regulates the Expression of Nmda Receptor Subunits Glun2a and Glun2b

This dissertation, "The X-encoded Nucleosome Assembly Protein Tspyl2 Regulates the Expression of NMDA Receptor Subunits GluN2A and GluN2B" by Ka-hing, Tsang, 曾家慶, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Since GluN2A and GluN2B are important in brain function, electrophysiological tests and behavioral tests were performed to study the phenotype of Tspyl2 mutant mice. Tspyl2 deficiency impaired hippocampal long-term potentiation in electrophysiological tests, indicating defects in long term plasticity. Tspyl2 mutant mice also showed defects in fear associated memory with Pavlovian fear conditioning tests but not in spatial memory with a conventional water-maze paradigm. To elucidate the signaling pathway involved, phosphorylation of downstream targets of NMDA receptors, Erk1/2 and p38 MAP kinase, was examined. Tspyl2 mutant hippocampus had a reduced level of phosphorylated p38 MAPK. These data suggested that Tspyl2 is important in brain function. Another member of NAP/SET/TSPY superfamily, Nap1l2, has been shown to regulate gene expression through promoting histone acetylation activity in neurons. To gain insight into the mechanism of how TSPYL2 regulates transcription, the effect of TSPYL2 on histone post-translational modifications was examined. H3K27Me2, a transcriptional repressive mark, was specifically increased in Tspyl2 mutant hippocampus and decreased in HEK293 cells overexpressing HA-TSPYL2. From immunocytochemistry, the staining pattern of H3K27Me2 in Tspyl2 mutant primary hippocampal neurons and HA-TSPYL2 transfected HEK293 cells was altered. These data suggested that Tspyl2 reduces the level of H3K27Me2. In conclusion, the present study shows that Tspyl2 is important in neuronal function through transcription regulation. Tspyl2 regulated the expression of Grin2a and Grin2b in hippocampal neurons. In addition, Tspyl2 reduced the di-methylation on histone H3 lysine 27 residues which is likely to affect the expression of a number of genes. Loss-of-function of Tspyl2 impaired learning and memory. DOI: 10.5353/th_b5016251 Subjects: Methyl aspartate - Receptors Nucleoproteins