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Studies from the Rockefeller Institute for Medical Research Volume 12

Studies from the Rockefeller Institute for Medical Research Volume 12


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This historic book may have numerous typos and missing text. Purchasers can download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1911 Excerpt: ...amount of autolysis in acid, neutral and alkaline media. Inasmuch as inhibition of autolysis has been observed in expressed tissue juices after thorough perfusion, it is manifestly impossible to free tissue from all inhibiting substances. Experiments V and VI represent two different types of autolytic activity occurring in normal liver. In both instances what is probably the maximal amount of proteolysis is developed in acid medium. In Experiment V the activity of digestion sinks markedly in alkaline medium, and when a concentration of 0.4 per cent, sodium carbonate is reached, no proteolysis occurs. On the other hand, in Experiment VI a second.optimum of digestion occurs in alkaline medium and this high degree of autolytic activity is maintained even in a concentration of 0.4 per cent, sodium carbonate. The latter type of activity is rarely observed in autolysis of normal liver. A similar observation has been reported by Baer and Loeb.23 Inasmuch as it will be shown later that liver contains a masked alkaline enzyme whose activity can be developed by appropriate methods, the result obtained in Experiment VI suggests that the activity of this enzyme is subject to marked variations during life. An attempt was made to duplicate, in two additional experiments, the second optimum of activity in alkaline media, which I have described above. Normal liver was allowed to autolyze in strengths of acid and alkali varying from one per cent, acetic acid to one per cent, sodium carbonate. "Baer and Loeb, loc. cit. Experiment VIII.--The liver of a normal dog with no perfusion was excised aseptically and rapidly hashed. Denaturalized beef serum was added as substrate; the duration of digestion was five days at 37C. The results obtained in these two instances are prac...


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Product Details
  • ISBN-13: 9781231657102
  • Publisher: Rarebooksclub.com
  • Publisher Imprint: Rarebooksclub.com
  • Height: 246 mm
  • No of Pages: 194
  • Spine Width: 10 mm
  • Width: 189 mm
  • ISBN-10: 1231657103
  • Publisher Date: 01 May 2012
  • Binding: Paperback
  • Language: English
  • Returnable: N
  • Weight: 354 gr


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