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Home > Art, Film & Photography > Sulfonyl Halides: Dansyl Chloride, 4-Toluenesulfonyl Chloride, Pmsf, Sulfonyl Halide
Sulfonyl Halides: Dansyl Chloride, 4-Toluenesulfonyl Chloride, Pmsf, Sulfonyl Halide

Sulfonyl Halides: Dansyl Chloride, 4-Toluenesulfonyl Chloride, Pmsf, Sulfonyl Halide


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About the Book

Chapters: Dansyl Chloride, 4-Toluenesulfonyl Chloride, Pmsf, Sulfonyl Halide. Source: Wikipedia. Pages: 20. Not illustrated. Free updates online. Purchase includes a free trial membership in the publisher's book club where you can select from more than a million books without charge. Excerpt: 70 C, 343 K, 158 F Dansyl chloride or 5-(dimethylamino)naphthalene-1-sulfonyl chloride is a reagent that reacts with primary amino groups in both aliphatic and aromatic amines to produce stable blue- or blue-greenfluorescent sulfonamide adducts. Dansyl chloride is widely used to modify amino acids; specifically, protein sequencing and amino acid analysis. Dansyl chloride may also be denoted DNSC. Likewise, a similar derivative, dansyl amide is known as DNSA. In addition, these protein-DNSC conjugates are sensitive to their immediate environment. This, in combination with their ability to accept energy (as in fluorescence resonance energy transfer) from the amino acid tryptophan, allows this labeling technique to be used in investigating protein folding and dynamics. The fluorescence of these sulfonamide adducts can be enhanced by adding cycloheptaamylose. Dansyl chloride is unstable in dimethyl sulfoxide, which should never be used to prepare solutions of the reagent. The extinction coefficient of dansyl derivatives are important for measuring their concentration in solution. Dansyl chloride is one of the simplest sulfonamide derivatives, so it commonly serves as a starting reagent for the production of other derivatives. Exotic derivatives may have very different extinction coefficients, but others, such as dansyl amide, are similar to dansyl chloride in absorption and fluorescence characteristics. But even for dansyl chloride, there are a variety of extinction coefficient values that have been reported. Some of the values are used to estimate the extent of success in attempts to conjugate the dye to a protein. Other values may be ...More: http: //booksllc.net/?id=13573006


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Product Details
  • ISBN-13: 9781158648351
  • Publisher: Books LLC
  • Publisher Imprint: Books LLC
  • Height: 152 mm
  • No of Pages: 22
  • Spine Width: 1 mm
  • Weight: 45 gr
  • ISBN-10: 1158648359
  • Publisher Date: 15 Sep 2010
  • Binding: Paperback
  • Language: English
  • Returnable: N
  • Sub Title: Dansyl Chloride, 4-Toluenesulfonyl Chloride, Pmsf, Sulfonyl Halide
  • Width: 229 mm


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