The book brings together all important principles of nonlinear optical (NLO) microscopy. By presenting NLO microscopy within a consistent foundational framework, the origin of the signals and the interrelation between different NLO techniques can be understood. The book provides rigorous yet practical derivations, which amount to expressions that can be directly related to measured values of resolution, imaging speed, sensitivity and imaging contrast. The book addresses typical questions users and students ask and answers them with clear explanations and examples. Readers of this book will develop a solid physical understanding of NLO microscopy, appreciate the advantages and limitations of each technique, and recognize the exciting possibilities that lie ahead.
Table of Contents:
Introduction
Chapter 1. Light Propagation
Chapter 2. Focusing of Light
Chapter 3. Pulses of Light
Chapter 4. Classical Description of Light-Matter Interactions
Chapter 5. Quantum Mechanical Description of Light-Matter Interactions
Chapter 6. Molecular Excitations
Chapter 7. Signal Radiation
Chapter 8. Detection of Light
Chapter 9. Harmonic Generation
Chapter 10. Sum-frequency Generation
Chapter 11. Coherent Raman Scattering
Chapter 12. Pump-Probe Microscopy
Chapter 13. Light Scattering in Tissues
Chapter 14. Super-resolution Nonlinear Microscopy
Chapter 15. Near-Field and Surface-Enhanced Nonlinear Microscopy
Appendices
Appendix I: Molecular Vibrations, Symmetry and Selection Rules
Appendix II: Mode-Coupling and Fermi Resonances
About the Author :
Eric Olaf Potma is Professor at the University of California, Irvine, in the Department of Chemistry. His research interests are quantitative imaging with nonlinear optical microscopy, nonlinear optics of individual molecules and nanostructures, and nonlinear optical scan probe microscopy.
