Protein Targeting
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Protein Targeting: A Practical Approach(101 Practical Approach Series)

Protein Targeting: A Practical Approach(101 Practical Approach Series)

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About the Book

It has been known for many years that the subcellular distribution of proteins throughout eukaryotic cells is highly ordered. This means that proteins have to be transported to their correct destinations and left there. Understanding the complex mechanisms by which this occurs has provided a major challenge for modern biology. Recent progress in the subject has relied heavily on finding ways to measure protein transport and targeting. Protein Targeting brings together contributions from active researchers in the field, who provide detailed step-by-step protocols for studying the processes involved, using both intact cells and broken cell preparations. Cell biological, biochemical, and molecular biological methods are all addressed in detail, and the chapters cover the well-established techniques, as well as a number of exciting new methodologies which have potentially very wide applications. This book will enable scientists from varied backgrounds to get to grips with unravelling the mechanisms which control cellular protein targeting.

Table of Contents:
C.M. Nolan: Receptor-mediated endocytosis and lysosomal transport; J.A. Ellis, M.R. Jackman, J.H. Perez, B.M. Mullock, J.P. Luzio: Membrane traffic pathways in polarized epithelial cells; L.P. Cramer & D.F. Cutler: Sorting between exocytic pathways in PC12 cells; J. Armstrong, E. Fawell, & A. Pidoux: Intracellular trafficking in fission yeast; D. Vaux: Network antibodies as tools for studying intracellular protein traffic; U. Wienhues, H. Koll, K. Becker, B. Guiard, & F-U. Hartl: Protein targeting to mitochondria; C. Dingwall: Techniques in nuclear protein transport; J. Gruenberg, & J.P. Gorvel: In vitro reconstruction of endocytic vesicle fusion; R. Schwaninger: In vitro reconstitution of vesicular transport from the endoplasmic reticulum to the cis Golgi in semi-intact cells; W.J. Masterson, & A.I. Magee: Lipid modifications involved in protein targeting.


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Product Details
  • ISBN-13: 9780199632107
  • Publisher: Oxford University Press
  • Publisher Imprint: Oxford University Press
  • Height: 234 mm
  • No of Pages: 288
  • Sub Title: A Practical Approach
  • Width: 156 mm
  • ISBN-10: 0199632103
  • Publisher Date: 20 Aug 1992
  • Binding: Hardback
  • Language: English
  • Series Title: 101 Practical Approach Series
  • Weight: 499 gr


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