Eukaryotic Cell Genetics

Eukaryotic Cell Genetics reviews the state of knowledge in somatic cell genetics. The book begins by discussing the development of somatic cell genetics, focusing on the estimation of mutation rates in mammalian cells, with frequent reference to the use of drug resistance as a selective character. It then considers some of the specific properties of such variants in order to understand their molecular basis. The subsequent chapters examine the properties of specific types of auxotrophic variants; the means by which eukaryotic cells may be reassembled to give rise to viable cellular composites; gene regulation in eukaryotic organisms; and chromosome mapping. The discussions also include differentiation in cultured cells; neoplastic transformation; the modulation of gene expression in cultured cells; mutation induction in cultured cells; applications of cell culture; and the mechanism of cellular aging. This book is intended for researchers in the fields of genetics and molecular biology, nonspecialists interested in what is happening in a very exciting area of biology, and students at the graduate level in cell biology.

Table of Contents:
Preface1 Somatic Cell Genetics and the Legacy of Microbial Systems I. Introduction II. Mutation versus Adaptation in Bacterial Populations III. The Basis of Variation in Somatic Cells IV. Summary and Conclusions2 Drug Resistance and Its Genetic Basis I. Introduction II. Purine Analogs III. Pyrimidine Analogs IV. Drugs Other than Purine and Pyrimidine Analogs V. Conclusions3 Auxotrophic Variants in Cultured Cells I. Introduction II. Techniques for Isolation III. Properties of Variants IV. Conclusions4 Mechanisms for the Exchange of Genetic Information in Cultured Cells I. Introduction II. Cell Hybridization III. Genetic Exchange Using Cell Components IV. Transfer of Genetic Information Using Purified Metaphase Chromosomes V. Transformation Using Purified DNA Preparations VI. Transfer of Information through Direct Microinjection of RNA and DNA VII. Conclusion5 The Regulation of Gene Expression in Heterokaryons I. Introduction II. Nuclear Reactivation in Heterokaryons III. The Expression of Genetic Information in Heterokaryons IV. The Mechanism of Nuclear Activation in Heterokaryons V. Conclusions6 Chromosome Mapping I. Introduction II. Mapping by Pedigree Analysis III. Linkage Mapping Using Somatic Cell Hybridization, Chromosomal Variants, and Nucleic Acid Hybridization IV. Conclusions7 Differentiation in Cultured Cells: Liver Cells I. Introduction II. Liver Cells and Their Hybrids in the Study of Differentiation III. Clonal Variation in Liver Cells IV. Transfer of Control Factors via Cytoplasms V. Conclusion8 Differentiation in Cultured Cells: Muscle Cells, Melanoma Cells, Neuronal Cells, and Hemoglobin- Producing Cells I. Introduction II. Muscle III. Melanin Synthesis in Cell Hybrids IV. Nerve Cells V. Hemoglobin Synthesis in Cell Hybrids VI. Conclusion and Summary9 Differentiation in Cultured Cells: Cells of the Immune System I. Introduction II. Mechanism of Antibody Diversity III. The Structure of the Antibody Molecule IV. The Arrangement of Antibody Genes V. Immunoglobulin Expression in Cell Hybrids VI. Monoclonat Antibodies Produced by Cell Hybrids VII. Conclusion10 Hypotheses of Malignancy and Their Analysis through the Use of Somatic Cell Hybrids I. Introduction II. Models of Cancer III. Analysis of Malignancy in Intraspecific Somatic Cell Hybrids IV. Genetic Control of Malignancy in Interspecific Hybrids V. Chromosomal Alterations and Malignancy VI. Conclusions11 Modulation of Gene Expression in Cultured Cells I. Introduction II. Response to Steroid Hormones in Eukaryotic Cells III. Effects of Bromodeoxyuridine on Differentiated Gene Functions IV. Cyclic AMP Response in Cultured Cells V. Conclusions12 Mutation Induction in Cultured Cells I. Introduction II. Problems Involved in Mutagenesis Studies III. Induction of Mutations by X Rays and Nonionizing Radiation IV. Chemical Mutagenesis V. Conclusions13 Use of Cell Culture in the Analysis of Human Heredity I. Introduction II. Hypercholesterolemia III. X Chromosomal Inactivation IV. Xeroderma Pigmentosum V. Testicular Feminization VI. Conclusions14 The Cellular Basis of the Aging Process I. Introduction II. The Aging of Diploid Fibroblasts in Vitro III. Proliferative Capacity of Diploid Fibroblasts and the Normal Aging Process IV. Error Catastrophe Hypothesis V. Conclusions15 Future Outlook I. Introduction II. Recombinant DNA Technology III. Immunogenetics IV. Human Genetics V. Aging VI. Malignancy VII. Differentiation VIII. ConclusionsBibliographyIndex