Skeletal Development and Repair
Home > Medicine & Health Science textbooks > Medicine: general issues > Medical equipment and techniques > Medical research > Skeletal Development and Repair: Methods and Protocols
Skeletal Development and Repair: Methods and Protocols

Skeletal Development and Repair: Methods and Protocols

|
     0     
5
4
3
2
1




International Edition


About the Book

This second edition provides a comprehensive laboratory manual on skeletal development and skeletal repair research utilizing mouse models. Chapters provide methods and protocols on the most current and cutting-edge techniques in skeletal development and repair, histological, cellular, and molecular techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Skeletal Development and Repair: Methods and Protocols-Second Edition aims to ensure successful results in the further study of this vital field.

Table of Contents:
 Overview of Skeletal Development.- Overview of Skeletal Repair (Fracture Healing and Its Assessment).- Advantages and Limitations of Cre Mouse Lines Used in Skeletal Research.- Generation of Closed Transverse Fractures in Small Animals.- A Mouse Femoral Ostectomy Model to Assess Bone Graft Substitutes.- Surgical Induction of Posttraumatic Osteoarthritis in the Mouse.- Parabiosis: Assessing the Effects of Circulating Cells and Factors on the Skeleton.- Murine Limb Bud Organ Cultures for Studying Musculoskeletal Development.- Murine Limb Explant Cultures to Assess Cartilage Development.- Renal Capsule Transplantation to Assay Angiogenesis in Skeletal Development and Repair.- MicroCT for Scanning and Analysis of Mouse Bones.- Four-Point Bending Testing for Mechanical Assessment of Mouse Bone Structural Properties.- Phenotyping Intact Mouse Bones using Bone CLARITY.- Processing and Sectioning Undecalcified Murine Bone Specimens.- Preparation of Thin Frozen Sections from Nonfixed And Undecalcified Hard Tissues Using Kawamoto's Film Method (2020).- Demineralized Murine Skeletal Histology.- Assessment of Osteocytes: Techniques for Studying Morphological and Molecular Changes Associated With Perilacunar/ Canalicular Remodeling Of the Bone Matrix.- Cell Lineage Tracing: Co-localization of Cell Lineage Markers with a Fluorescent Reporter.- Immunofluorescent Staining of Adult Murine Paraffin-embedded Skeletal Tissue.- Detection of Hypoxic Regions in the Bone Microenvironment.- EdU-based Assay of Cell Proliferation and Stem Cell Quiescence in Skeletal Tissue Sections.- Whole-mount In Situ Hybridization in Murine Tissues.- Bone Marrow Stromal Cell Assays – In Vitro and In Vivo.- Isolation and Culture of Periosteum Derived Progenitor Cells from Mice.- Isolation and Culture of Murine Primary Chondrocytes: Costal and Growth Plate Cartilage.- Isolation and Culture of Neonatal Mouse Calvarial Osteoblasts.-Mitochondrial Function and Metabolism of Cultured Skeletal Cells.- Radiolabeled Amino Acid Uptake Assays in Primary Bone Cells and Bone Explants.- RANKL-based Osteoclastogenic Assay from Murine Bone Marrow Cells.- Hematopoietic Stem Cell Cultures and Assays. 


Best Sellers


Product Details
  • ISBN-13: 9781071610275
  • Publisher: Springer-Verlag New York Inc.
  • Publisher Imprint: Springer-Verlag New York Inc.
  • Edition: Revised edition
  • Language: English
  • Returnable: Y
  • Sub Title: Methods and Protocols
  • ISBN-10: 1071610279
  • Publisher Date: 17 Nov 2020
  • Binding: Hardback
  • Height: 254 mm
  • No of Pages: 482
  • Returnable: Y
  • Width: 178 mm


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
Skeletal Development and Repair: Methods and Protocols
Springer-Verlag New York Inc. -
Skeletal Development and Repair: Methods and Protocols
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

Skeletal Development and Repair: Methods and Protocols

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept

    New Arrivals

    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!