Quantitative Methods in Proteomics
Home > Mathematics and Science Textbooks > Biology, life sciences > Biochemistry > Quantitative Methods in Proteomics
Quantitative Methods in Proteomics

Quantitative Methods in Proteomics

|
     0     
5
4
3
2
1




International Edition


About the Book

This second edition provides new and updated methods on the principles underlying modern protein analysis, from statistical issues to gel-based and mass spectrometry-based applications. Chapters detail protein quantification as basis for realisation of quantitative studies, gel-based and mass spectrometry-based quantification techniques, TMT, IPTL, PRM, MALDI Imaging, SILAC, PTM analysis, DIA, cross-linking, and the up-to-date topics of software and data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and cutting-edge,  Quantitative Methods in Proteomics, Second Edition aims to provide comprehensive and competent overview in the important and still growing field of quantitative proteomics.

Table of Contents:
Important Issues in Planning a Proteomics Experiment: Statistical Considerations of Quantitative Proteomic Data.- Good Old-Fashioned Protein Concentration Determination by Amino Acid Analysis.- Protein Quantification using the “Rapid Western Blot” Approach.- The Whereabouts of 2D Gels in Quantitative Proteomics.- Proteome Analysis with Classical 2D‑PAGE.- Silver Staining of 2D Electrophoresis Gels.- Differential Proteome Analysis using 2D-DIGE.- Quantitative Mass Spectrometry-Based Proteomics: An Overview.- Tandem Mass Tags for Comparative and Discovery Proteomics.- An Approach for Triplex-IPTL.- Targeted Protein Quantification Using Parallel Reaction Monitoring (PRM).- Quantitative Approach using Matrix Assisted Laser Desorption/Ionization Time of Flight (MALDI-ToF) Mass Spectrometry.- Application of SILAC Labeling in Phosphoproteomics Analysis.- Relative Quantification Of Phosphorylated and Glycosylated Peptides from the Same Sample using Isobaric Chemical Labelling with a Two-step Enrichment Strategy.- High Throughput Profiling of Proteome and Posttranslational Modifications by 16-plex TMT Labeling and Mass Spectrometry.- Quantification and Identification of Post-Translational Modifications using Modern Proteomics Approaches.- Affinity-enrichment Chemoproteomics for Target Deconvolution and Selectivity Profiling.- 2nSILAC for Quantitative Proteomics of Prototrophic Baker's Yeast.- Metabolic Labeling of Clostridioides difficile Proteins.- Application of Label-Free Proteomics for Quantitative Analysis of Urothelial Carcinoma and Cystitis Tissue.- Quantitative MS Workflow for a High-Quality Secretome Analysis by a Quantitative Secretome-proteome Comparison.- Establishing a Custom-Fit Data Independent Acquisition Method for Label Free Proteomics.- Label-free Proteomics Of Quantity-Limited Samples Using Ion Mobility-Assisted Data-Independent Acquisition Mass Spectrometry.- DIA-MSEto Study Microglial Function in Schizophrenia.- Detailed Method for Performing the ExSTA Approach in Quantitative Bottom-up Plasma Proteomics.- Quantitative Cross-Linking of Proteins and Protein Complexes.- Missing Value Monitoring to Address Missing Values in Quantitative Proteomics.- Quantitative Proteome Data Analysis of Tandem Mass Tags Labeled Samples.- Mining Protein Expression Databases Using Network Meta-Analysis.- A Tutorial for Variance-sensitive Clustering And The Quantitative Analysis Of Protein Complexes.- Automated Workflow for Peptide-level Quantitation from DIA/ SWATH-MS Data.   


Best Sellers


Product Details
  • ISBN-13: 9781071610237
  • Publisher: Springer-Verlag New York Inc.
  • Publisher Imprint: Springer-Verlag New York Inc.
  • Edition: Revised edition
  • Language: English
  • Returnable: Y
  • Width: 178 mm
  • ISBN-10: 1071610236
  • Publisher Date: 06 May 2021
  • Binding: Hardback
  • Height: 254 mm
  • No of Pages: 483
  • Returnable: Y


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
Quantitative Methods in Proteomics
Springer-Verlag New York Inc. -
Quantitative Methods in Proteomics
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

Quantitative Methods in Proteomics

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept

    New Arrivals

    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!