Protein Phosphatase Protocols
Home > Mathematics and Science Textbooks > Biology, life sciences > Biochemistry > Protein Phosphatase Protocols
Protein Phosphatase Protocols

Protein Phosphatase Protocols

|
     0     
5
4
3
2
1




International Edition


About the Book

A major mechanism by which cells regulate protein function is to place phosphate groups on serine and threonine residues. Though the steady-state level of protein phosphorylation depends on the relative activities of both kinases and phosphatases, a much greater effort has previously gone into the study of the former that the latter . Today, however, there is an increasing appreciation for the role that protein phosphatases play in the dynamic p- cess of protein phosphorylation . To date, there are four major types of protein serine/threonine phosphatase catalytic subunits, designated protein phosphatase type 1, 2A, 2B, and 2C . Each has been identified by the techniques of protein chemistry and enzymology and can be distinguished from one another by their preference for specific substrates as well as their sensitivity to certain acti- tors and inhibitors . Protein Phosphatase Protocols has been assembled in response to the growing interest these enzymes are receiving . The goal of this compilation is to provide a "how-to" experimental guide to aid newcomers as well as s- soned veterans in their research endeavors, thus further contributing towards our ever increasing knowledge of serine/threonine phosphatases . What you have before you contains contributions by many of the current and emerging leaders in the field . To highlight just a few, these chapters c- tain step-by-step information on how to isolate novel phosphatases and re- latory subunits, assay for activity, and generate immunological reagents for both biochemical and biological characterization of these enzymes .

Table of Contents:
Prokaryotic Protein-Serine/Threonine Phosphatases.- Protein Phosphatase Type 1 and Type 2A Assays.- Analyzing Gene Expression with the Use of Serine/Threonine Phosphatase Inhibitors.- Inhibitor-1, a Regulator of Protein Phosphatase 1 Function.- I1 PP2A and I2 PP2A.- Control of PP1 Activity Through Phosphorylation by Cyclin-Dependent Kinases.- Regulation of Neuronal PP1 and PP2A During Development.- PTPA Regulating PP2A as a Dual Specificity Phosphatase.- Microinjection and Immunological Methods in the Analysis of Type 1 and 2A Protein Phosphatases from Mammalian Cells.- Use of Immunocomplexed Substrate for Detecting PP1 Activity.- The Biochemical Identification and Characterization of New Species of Protein Phosphatase 1.- The Relationship Between Insulin Signaling and Protein Phosphatase 1 Activation.- Analysis of the Isoforms of Protein Phosphatase 1 (PP1) Isoforms with Polyclonal Peptide Antibodies.- Expression of Mouse Protein Phosphatase 2C in Eschericia coli and COS 7 Cells.- Expression of Functional Protein Phosphatase 1 Catalytic Subunit in E. coli.- Protein Phosphatase 2A and Protein Phosphatase X Genes in Arabidopsis thaliana.- Separation of Protein Phosphatase Type 2C Isozymes by Chromatography on Blue Sepharose.- Chromatographic Isolation of PP2A from Limulus Lateral Eyes.- Purification and Assay of the Ptc/Tpd1 Protein Phosphatase 2C from the Yeast Saccharomyces cerevisiae.- Molecular Cloning of Protein Phosphatase Type 2C Isoforms from Retinal cDNA.- Analysis of Protein Interactions Between Protein Phosphatase 1 and Noncatalytic Subunits Using the Yeast Two-Hybrid Assay.- Identifying Protein Phosphatase 2A Interacting Proteins Using the Yeast Two-Hybrid Method.- Protein Phosphatase 2A Regulatory Subunits.- Synthetic Lethal Screening in Protein PhosphatasePathways.- The Search for the Biological Function of Novel Yeast Ser/Thr Phosphatases.


Best Sellers


Product Details
  • ISBN-13: 9781489942760
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Height: 229 mm
  • No of Pages: 316
  • Width: 152 mm
  • ISBN-10: 1489942769
  • Publisher Date: 14 Aug 2013
  • Binding: Paperback
  • Language: English
  • Returnable: Y


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
Protein Phosphatase Protocols
Humana Press Inc. -
Protein Phosphatase Protocols
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

Protein Phosphatase Protocols

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept

    New Arrivals

    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!