Nitric Oxide: Methods and Protocols

This volume explores three main aspects of nitric oxide (NO) research: NO treatment and detection, NO modifications, and NO detoxification. The book also covers methods used to study human/animal and plant nitric oxide. The chapters are divided into three parts: part one looks at NO treatments using gaseous nitric oxide and detection using a NO-sensitive electrode, electron spin resonance, and fluorescence-based NO-sensor proteins. Part two talks about various techniques used to detect and identify NO-dependent modifications, such as biotin-switch assay and quantification of s-nitrosated proteins. Part three focuses on the study of s-nitrosothiol homeostasis and denitrosation activities. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and authoritative, Nitric Oxide: Methods and Protocols is a valuable resource for anyone interested in learning more about this evolving field.

Table of Contents:
A Simple and Useful Method to Apply Exogenous NO Gas to Plant Systems -   Bell Pepper Fruits as a Model.- Measurements of Intra-Oocyte Nitric Oxide Concentration Using Nitric Oxide Selective Electrode.- Real-Time Imaging of Nitric Oxide Signals in Individual Cells Using geNOps.- Detection of Nitric Oxide by Membrane Inlet Mass Spectrometry.- Quantum Cascade Lasers-Based Detection of Nitric Oxide.- Detection of Nitric Oxide via Electronic Paramagnetic Resonance in Molluscs.- Identification of S-Nitrosylated and Reversibly Oxidized Proteins by Fluorescence Switch and Complementary Techniques.- A Proteomics Workflow for Dual Labeling Biotin Switch Assay to Detect and Quantify Protein S-Nitroylation.- Surface Plasmon Resonance Spectroscopy for Detection of S-Nitrosylated Proteins.- Measurement of S-Nitrosoglutathione in Plasma by Liquid Chromatography-Tandem Mass Spectrometry.- Analysis of Recombinant Protein S-Nitrosylation using the Biotin-Switch Technique.- Direct Measurement of S-Nitrosothiols with an Orbitrap Fusion Mass Spectrometer: S-Nitrosoglutathione Reductase as a Model Protein.- Identification of Tyrosine and Nitrotyrosine with a Mixed-Mode Solid-Phase Extraction Cleanup followed by Liquid Chromatography-Electrospray Time-of-Flight Mass Spectrometry in Plants.- Electrophoretic Detection and Confocal Microscopic Imaging by Tyrosine Nitrated Proteins in Plant Tissue.- Identification of NO-Sensitive Cysteine Residues using Cysteine Mutants of Recombinant Proteins.- Detection of S-Nitrosylated Nuclear Proteins in Pathogen-Treated Arabidopsis Cell Cultures using Biotin Switch Technique.- Nitric Oxide Analyzer Quantification of Plant S-Nitrosothiols.- Nitro-Fatty Acid Detection in Plants by High-Pressure Liquid Chromatography Couples to Triple Quadrupole Mass Spectrometry.- Alche_Bioinformatic Prediction of S-Nitrosylation Sites in Large Protein Datasets.- Biotin Switch Processing and Mass Spectrometry Analysis of S-Nitrosated Thioredoxin and its Transnitrosation Targets.- Immunodetection of S-Nitrosoglutathione Reductase Protein in Plant Samples.- Thioredoxin-Dependent Decomposition of Protein S-Nitrosothiols.