Neuroscience Protocols - Module 3

Neuroscience Protocols (NP) is a laboratory manual which provides protocols of new and established techniques used in neuroscience. The protocols are supplied in a loose-leaf format, with a ring binder. This makes it easy to remove the protocol you are using, or replace it with an update. NP is a compilation of modules. Each module contains about 10 - 12 new or updated protocols covering several subjects. Module I was published in May 1993; Module 2 in December 1993. Initially two modules will be published each year. Each new module contains updated cumulative contents of modules already published. The contents of the modules are available on request. Definitive contents are not known as yet. A ringbinder with sectional dividers is part of the Module I and 2 set and is also available separately. NP is divided into the following sections: Behavioural Neurobiology, Cellular and Molecular Neurobiology, Developmental Biology, Neuroimmunology, Neuromorphology, Neuropharmacology and Neurochemistry, Neurophysiology, and Quantitative Neurobiology. The Managing Editor of NP is F.G. Wouterlood who is based at the Department of Anatomy, Free University, Amsterdam, The Netherlands. The Editorial Board consists of eight neuroscientists who each have different areas of expertise. They are: J.F Batty, S.B. Dunnett, S. Hockfield, J.P. Houston, R.R. Mize, O.P. Ottersen, T.F. Robinson and C. Wilson. For more detailed information, please contact the Publisher. The first NP CD-ROM is scheduled for publication in 1995. The electronic version will include most of the protocols published so far. There will be a single-user version and a network version. The price for the CD-ROM is not yet known. However, customers with a standing order to the printed modules of NP will receive a significant reduction on the price of the CD-ROM.

Table of Contents:
Supplement 3. Guide for Using Neuroscience Protocols. Instructions to Authors. Section 010 - Behavioural Neurobiology. TRH-induced behaviour in rabbits as a simple model for pharmacological studies on the dopamine system. The shock-probe burying test. Paw reaching in rats: the staircase test. Section 020 - Cellular and Molecular Neurobiology. Preparation of biotinylated primary antibodies and their application for immunocytochemical dual-label experiments using antibodies from the same animal species. Section 030 - Developmental Neurobiology. Bromodeoxyuridine as a label for foetal hypothalamic whole tissue and cell suspension grafts. A device for single-cell transplantation with minimal transfer of suspension fluid. Section 040 - Neuroimmunology. Section 050 - Neuromorphology. Preparation, staining and examination of nervous tissue in the confocal scanning laser microscope. Anterograde and retrograde neuroanatomical tract tracing with fluorescent compounds. Combination of Golgi silver-staining and immunofluorescent techniques. Intracellular injection of transport markers and antibodies into in vitro cultured nerve cells. Intensification of signals in immunostaining, in situ hybridization, ligand binding and neuroanatomical tracing. Section 060 - Neuropharmacology and Neurochemistry. Construction and implantation of a chronic intracranial dialytic delivery system. Section 070 - Neurophysiology. Section 080 Quantitative Neurobiology. Computerized stereology: quantitative in situ hybridization.