Methods in Neurosciences
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Methods in Neurosciences

Methods in Neurosciences

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About the Book

Receptors initiate the means by which cellular regulators exert their actions on target cells. Because of the central role of cell-cell communication and signal transduction, receptors are of intrinsic interest to neuroscientists. Receptor studies utilize both traditional methods of analysis and modern molecular techniques.

Table of Contents:
Part 1 Molecular Biology (Molecular Cloning, PCR, Transcription, and Transfection): Methods for Studying the Control of Receptor Gene Expression - Promoter Mapping and Analysis of transcription Regulation, C.A. Harrington and N.J. Buckley; Cloning of G-Protein Coupled Receptors, C. Eva and R. Sprengel; Molecular Cloning and Expression of a D1 Dopamine Receptor, F.J. Monsma Jr and D.R. Sibley; Molecular Cloning of a Third Dopamine Receptor (D3), P. Sokoloff, et al; Molecular Biology of the Human Tachykinin Receptors, N.P. Gerard, et al; Purification and Molecular Cloning of the Bombesin/Gastrin-Releasing Peptide Receptor, J.F. Battey, et al; Expression and Characterization of Recombinant Nerve Growth Factor Receptors - Selection of Recombinant Baculoviruses and Membrane Binding Assays, B.L. Hempstead, et al; Cloning and Analysis of Nicotinic Acetylcholine Receptor Gene Expression in Goldfish Retina during Optic Nerve Regeneration. V. Hieber and D. Goldman; Receptor Protein-Tyrosine Kinases in the Nervous System, Cloning and Expression of trkB, D.S. Middlemas; Transfection NOth Nerve Growth Factor (NGF) Receptor CDNA, H. Matsushima and E. Rogenmann; A Strategy to Analyze Thymic Transcription of the a-3 Nicotinic Neuronal Acetylcholine Receptor Subunit, M. Mihovilovic, et al; Absolute Quantitation of GABA Receptor Subunit mRNAs by Competitive PCR, D.R. Grayson, et al. Part 2 A Receptor Subclasses: Angiotensin 11 Receptor Subt,,-pes in the Central Nervous System, M.E. Hunt and J.K. Wamsley; Radioligand Binding Studies of the Three Major Classes of Neurokinin Receptors, R. Quirion and T.-V. Dam; Brain Serotonin Receptor Subtypes - Radioligand Binding Assays, Second Messenger, Ligand Autoradiography, and in Situ Hybridization Histochemistry, J.M. Palacios, et al; Methyllycacon'tine - A New Probe That Discriminates between Nicotonic Acetylcholine Receptor Subclasses, S. Wonnacott, et al. Part 3 Receptor Localization: Localization and Quantification of Brain Somatostatin Receptors by Light Microscopic Radioautography, J. Epelbaum and J. Bertherat; Neurotensin Receptors in the Central Nervous System - Anatomical Localization by in Vitro Autoradiograph-VI and in Situ Hybridization Histochemistry, F.J. Garcia-Ladona, et al; Combined in Situ Hybridization and immunocytochemical Studies of neuro transmitter Receptor RNA in Cultured Cells, S. Biirsztajn, et al; Autoradiographic Localizafion of PutafiveNeuronal Nicotinic Receptors Using Snake Venom Neurotoxins, R.H. Lohring and D.W. Schulz; Determination of Binding Sites for Neuropeptide Y Using Combined Autoradiography and Immunocytochemistry, E.P.C.T. de Rijk and E.W. Roubos; Progesterone and Estrogen Receptor Immunocytochemistry in the Central Nervous System, M. Warembourg and P.P. Poulain. Part 4 Ligan Design: N-a-Biotinylated Neuropeptide Y Analogs, A. Balasubramaniam, et al; Designing Selective Analogs for Peptide Receptors - Neuropeptide Y as an Example, J.L. Krstenansky.


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Product Details
  • ISBN-13: 9780121852733
  • Publisher: Elsevier Science Publishing Co Inc
  • Publisher Imprint: Academic Press Inc
  • Height: 230 mm
  • Weight: 1021 gr
  • ISBN-10: 0121852733
  • Publisher Date: 28 Nov 1997
  • Binding: Hardback
  • Language: English


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