LC-MS/MS in Proteomics
Home > Mathematics and Science Textbooks > Biology, life sciences > Biochemistry > LC-MS/MS in Proteomics: Methods and Applications
LC-MS/MS in Proteomics: Methods and Applications

LC-MS/MS in Proteomics: Methods and Applications

|
     0     
5
4
3
2
1




Out of Stock


Notify me when this book is in stock
About the Book

With the development of new quantitative strategies and powerful bioinformatics tools to cope with the analysis of the large amounts of data generated in proteomics experiments, liquid chromatography with tandem mass spectrometry (LC-MS/MS) is making possible the analysis of proteins on a global scale, meaning that proteomics can now start competing with cDNA microarrays for the analysis of whole genomes. In LC-MS/MS in Proteomics: Methods and Applications, experts in the field provide protocols and up-to-date reviews of the applications of LC-MS/MS, with a particular focus on MS-based methods of protein and peptide quantification and the analysis of post-translational modifications. Beginning with overviews of the use of LC-M/MS in protein analysis, the book continues with topics such as protocols for the analysis of post-translational modifications, with particular focus on phosphorylation and glycosylation, popular techniques for quantitative proteomics, such as multiple reaction monitoring, metabolic labelling, and chemical tagging, biomarker discovery in biological fluids, as well as novel applications of LC-MS/MS. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, LC-MS/MS in Proteomics: Methods and Applications presents the techniques and concepts necessary in order to aid proteomic practitioners in the application of LC-MS/MS to essentially any biological problem.

Table of Contents:
Overviews of Techniques and Approaches in LC-MS-Based Proteomics.- Approaches and Applications of Quantitative LC-MS for Proteomics and Activitomics.- Overview of Quantitative LC-MS Techniques for Proteomics and Activitomics.- Instrumentation for LC-MS/MS in Proteomics.- Bioinformatics for LC-MS/MS-Based Proteomics.- Analysis of Post-translational Modifications by LC-MS/MS.- Structural Analysis of Post-Translational Modifications.- In-Depth Analysis of Protein Phosphorylation by Multidimensional Ion Exchange Chromatography and Mass Spectrometry.- Mapping of Phosphorylation Sites by LC-MS/MS.- Analysis of Carbohydrates on Proteins by Offline Normal-Phase Liquid Chromatography MALDI-TOF/TOF-MS/MS.- Techniques for Quantitative LC-MS.- Selected Reaction Monitoring Applied to Quantitative Proteomics.- Basic Design of MRM Assays for Peptide Quantification.- Proteome-Wide Quantitation by SILAC.- Quantification of Proteins by iTRAQ.- Quantification of Proteins by Label-Free LC-MS/MS.- Analysis of Protein Complexes and Organelles.- Protocol for Quantitative Proteomics of Cellular Membranes and Membrane Rafts.- Organelle Proteomics by Label-Free and SILAC-Based Protein Correlation Profiling.- Mapping Protein–Protein Interactions by Quantitative Proteomics.- Analysis of Biological Fluids and Clinical Samples.- Analysis of Serum Proteins by LC-MS/MS.- Urinary Proteome Profiling Using 2D-DIGE and LC-MS/MS.- Analysis of Peptides in Biological Fluids by LC-MS/MS.- Novel Applications of LC-MS.- Quantification of Protein Kinase Activities by LC-MS.- A Protocol for Top-Down Proteomics Using HPLC and ETD/PTR-MS.


Best Sellers


Product Details
  • ISBN-13: 9781493957446
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Height: 254 mm
  • No of Pages: 357
  • Sub Title: Methods and Applications
  • ISBN-10: 1493957449
  • Publisher Date: 23 Aug 2016
  • Binding: Paperback
  • Language: English
  • Returnable: Y
  • Width: 178 mm


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
LC-MS/MS in Proteomics: Methods and Applications
Humana Press Inc. -
LC-MS/MS in Proteomics: Methods and Applications
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

LC-MS/MS in Proteomics: Methods and Applications

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept

    New Arrivals

    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!