An Introduction to Toxicogenomics

Since the advent of cDNA microarrays, oligonucleotide array technology, and gene chip analysis, genomics has revolutionized the entire field of biomedical research. A byproduct of this revolution, toxicogenomics is a fast-rising star within toxicological analysis. Gathering together leading authors and scientists at the forefront of the field, An Introduction to Toxicogenomics provides a comprehensive overview of this new discipline. With a focus on toxicology, it introduces the basic principles of microarray/oligonucleotide array-based genomic analysis and explains how it fits into the field of biomedical research. These discussions provide an overview to the actual mechanics of the analyses themselves and offer insights on handling and quality control. Then the book features an important section on the basics of data analysis and clustering methods such as genetic algorithms. Finally, it covers the application of expression profiling in the field of toxicology and addresses the two fundamental types of analysis in detail, with sections dedicated to both mechanistic and predictive studies. Although toxicogenomics promises fast, efficient techniques and information-rich data, much of its potential remains untapped. An Introduction to Toxicogenomics consolidates the concepts underlying the field to provide a solid foundation from which to begin your research endeavors.

Table of Contents:
FUNDAMENTALS OF EXPRESSION PROFILING ANALYSIS Preparation and Utilization of Microarrays, Sophie Wildsmith and Fiona Spence Comparative Studies Using cDNA versus Oligonucleotide Arrays, Jiang Li and Jeffrey A. Johnson Quality Control of Expression Profiling Data, Andrew Hill and Maryann Whitley An Introduction to Cluster Analysis, Frederick Immermann and Youping Huang EXPRESSION PROFILING AND TOXICOLOGY: THE ADVENT OF TOXICOGENOMICS Transcriptional Profiling in Toxicology, Julia Scheel, Marie-Charlotte von Brevern, and Thorsten Storck MODEL SYSTEMS IN TOXICOGENOMIC STUDIES Microarrays in Drug Metabolism and Toxicology: Rat Liver Responses to Prototypical CYP Inducers, David Gerhold, Jian Xu, and Thomas Rushmore Characterization of Hepatocytes and Their Use as a Model System in Toxicogenomics, Thomas K. Baker, Marnie A. Higgins, Mark. A. Carfagna, and Timothy P. Ryan MECHANISTIC TOXICOGENOMICS Molecular Signatures of Dioxin Toxicity, J. Kevin Kerzee, Craig R. Tomlinson, Jennifer L. Marlowe, and Alvaro Puga Toxicogenomic Dissection of the Antioxidant Response, Jiang Li and Jeffrey A. Johnson An Overview of Mechanistic Toxicogenomic Studies, Julia Scheel, Marie-Charlotte von Brevern, and Thorsten Storck PREDICTIVE TOXICOGENOMICS Gene Expression Profile Databases in Toxicity Testing, Robert T. Dunn II and Kyle L. Kolaja Predictive Toxicogenomics, Mark W. Porter, Arthur L. Castle, Michael S. Orr, and Donna L. Mendrick Unsupervised Hierarchical Clustering of Toxicants, Jeffrey F. Waring and Roger G. Ulrich Toxicogenomic Analysis in Clinical Phamacogenomic Studies, Michael E. Burczynski THE FUTURE OF TOXICOGENOMICS The Future of Toxicogenomics, John C. Rockett