Fluorescence Microscopy
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Fluorescence Microscopy

Fluorescence Microscopy

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About the Book

Fluorescence microscopy is used for studying the distribution of substances which are present in very small amounts. The high sensitivity of the method makes it ideal for studying the distribution of substances in living cells. Its techniques are used mainly in biology and medicine, but are also valuable in coal petrology and elsewhere. The best-known application is in immunofluorescence. This magnificent new work provides comprehensive coverage of all aspects of fluorescence microscopy. It covers instrumentation, applications to a wide variety of fields, and the history of the technique. There is a chapter on quantitative techniques, including scanning: this aspect is dealt with in more detail in a companion volume, Quantitative Fluorescence Microscopy. Volume 1 deals with instrumentation and techniques for fluorescence microscopy, and includes the chapter on quantitation and scanning. Volume 2 deals with the applications of fluorescence microscopy in many fields. It includes information on fluorochromes and on autofluorescence. An invaluable appendix provides an alphabetical list of fluorochromes, giving information concerning chemical structure, fluorescence properties, applications and suitable filter combinations. These two important volumes will be of use to all fluorescence microscopists and will be an invaluable reference tool for those graduate students and research workers in biology, medicine and earth science who need to make use of these techniques.

Table of Contents:
List of plates; Preface; Abbreviations and symbols; Principles of fluorescence microscopy; Fluorescence: physics and chemistry; The optical system of the fluorescence microscope; Commercial fluorescence microscopes; Setting up and using the fluorescence microscope; Photography of fluorescence; The interpretation of the fluorescence microscope image; Quantitative and scanning fluorescence microscopy; Appendices; German - English vocabulary; Checklist of features of fluorescence microscope; The laboratory microscope; Faults in the microscope and possible remedies; Data on photographic films for fluorescence photomicrography; Reciprocity failure; Relative brightness of image on film in various formats; Faults in photomicrographs and possible remedies; References; Index.


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Product Details
  • ISBN-13: 9780521236416
  • Publisher: Cambridge University Press
  • Publisher Imprint: Cambridge University Press
  • Height: 254 mm
  • No of Pages: 267
  • Returnable: N
  • Returnable: N
  • Weight: 817 gr
  • ISBN-10: 052123641X
  • Publisher Date: 05 Mar 1992
  • Binding: Hardback
  • Language: English
  • Returnable: N
  • Returnable: N
  • Spine Width: 19 mm
  • Width: 193 mm


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Fluorescence Microscopy
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