Current Protocols Essential Laboratory Techniques
Home > Mathematics and Science Textbooks > Biology, life sciences > Current Protocols Essential Laboratory Techniques
Current Protocols Essential Laboratory Techniques

Current Protocols Essential Laboratory Techniques

|
     0     
5
4
3
2
1




Out of Stock


Notify me when this book is in stock
About the Book

2008 PROSE award winner for Excellence in Biology and the Life Sciences From the leading branded source for methods in the life sciences, this essential resource for the lab provides every researcher with the skills and understanding of fundamental laboratory procedures to ensure greater success at the bench. It takes the novice researcher from the very basics of reagent preparation to the use of routine instrumentation found in most life science research laboratories around the world. Developed by Current Protocols , the most trusted and recognized source of protocols Teaches new investigators how to perform basic laboratory research techniques Describes solution chemistry and preparation Covers basic laboratory safety Describes care and use of common equipment such as pH meters, spectrophotometers, centrifuges, and microscopes Teaches how to manage information from lab notebooks, images, literature references, as well as manuscript preparation This book is an invaluable resource for researchers in all areas of the life sciences, especially molecular biology, biotechnology, genetics, and immunology. It is essential reading for undergraduate and graduate students alike and is also of interest for investigators new to life science research.

Table of Contents:
Foreword. Preface. Contributors. Common Conversion Factors. Combining Techniques to Answer Molecular Questions. Chapter 1: Volume/Weight Measurement. 1.1 Volume Measurement. 1.2 Weight Measurement. Chapter 2: Concentration Measurement. 2.1 Spectrophotometry. 2.2 Quantitation of Nucleic Acids and Proteins. 2.3 Radiation Safety and Measurement. Chapter 3: Reagent Preparation. 3.1 Reagent Preparation: Theoretical and Practical Discussions. 3.2 Measurement of pH. 3.3 Recipes for Commonly Encountered Reagents. Chapter 4: Cell Culture Techniques. 4.1 Aseptic Technique. 4.2 Culture of Escherichia coli and Related Bacteria. Chapter 5: Sample Preparation. 5.1 Centrifugation. 5.2 Purification and Concentration of Nucleic Acids. Chapter 6: Chromatography. 6.1 Overview of Chromatography. 6.2 Column Chromatography. Chapter 7: Electrophoresis. 7.1 Overview of Electrophoresis. 7.2 Agarose Gel Electrophoresis. 7.3 SDS-Polyacrylamide Gel Electrophoresis (SDS-PAGE). 7.4 Staining Proteins in Gels. 7.5 Overview of Digital Electrophoresis Analysis. Chapter 8: Blotting. 8.1 Overview of Blotting. 8.2 Nucleic Acid Blotting: Southern and Northern. 8.3 Protein Blotting: Immunoblotting. 8.4 Labeling DNA and Preparing Probes. Chapter 9: Microscopy. 9.1 Conventional Light Microscopy. 9.2 Immunofluorescence Microscopy. Chapter 10: Enzymatic Reactions. 10.1 Working with Enzymes. 10.2 Overview of PCR. 10.3 Real-Time PCR. 10.4 DNA Sequencing: An Outsourcing Guide. Appendices. A.1 Laboratory Safety. A.2 Laboratory Notebooks and Data Storage. A.3A Ethical Considerations When Altering Digital Images. A.3B Practical Considerations When Altering Digital Images. A.4 Statistical Analysis. A.5A Preparing and Presenting a Poster. A.5B Preparing and Presenting a Talk. Index.


Best Sellers


Product Details
  • ISBN-13: 9780470089934
  • Publisher: John Wiley and Sons Ltd
  • Binding: Paperback
  • Language: English
  • Returnable: N
  • Weight: 1809 gr
  • ISBN-10: 0470089938
  • Publisher Date: 06 Jun 2008
  • Height: 277 mm
  • No of Pages: 806
  • Spine Width: 37 mm
  • Width: 213 mm


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
Current Protocols Essential Laboratory Techniques
John Wiley and Sons Ltd -
Current Protocols Essential Laboratory Techniques
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

Current Protocols Essential Laboratory Techniques

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept

    New Arrivals

    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!