About the Book
The book will present the progress made since the last meeting in fall 2013 concerning the analysis of circulating extra-cellular nucleic acids. There are a modest number of laboratories involved in this field, nevertheless the number of papers published by researchers is extensive. The articles which will be published in this meeting report will be a valuable contribution for researchers and research students alike and will help them to stay on top of the developments in different research areas and to „cross borders“ between them.
Table of Contents:
Foreword Peter B. Gahan, Michael Fleischhacker, Bernd Schmidt .- Part I Nucleic Acids in Oncology – prognosis, treatments screening and metastases.- 1 Circulating Cell-free miR-373, miR-200a, miR-200b and miR-200c in Patients with Epithelial Ovarian Cancer Xiaodan Meng, Volkmar Müller, Karin Milde-Langosch, Fabian Trillsch, Klaus Pantel, Heidi Schwarzenbach.- 2 Cell-free miRNA-141 and miRNA-205 as Prostate Cancer Biomarkers Ivan D. Osipov, Ivan A. Zaporozhchenko, Anna A. Bondar, Marat M. Zaripov, Vladimir E. Voytsitskiy, Valentin V. Vlassov, Pavel P. Laktionov, Evgeny S. Morozkin.- 3 Clinical utility of circulating tumor DNA for molecular assessment and precision medicine in pancreatic cancer Erina Takai, Yasushi Totoki, Hiromi Nakamura, Mamoru Kato, Tatsuhiro Shibata, Shinichi Yachida.- 4 An Enquiry Concerning the Characteristics of Cell-free DNA Released by Cultured Cancer Cells Abel J Bronkhorst, JohannesF Wentzel, Janine Aucamp, Etresia van Dyk, Lissinda du Plessis, Piet J Pretorius .- 5 Detection of p53 Mutations in Circulating DNA of Transplanted Hepatocellular Carcinoma Patients as a Biomarker of Tumor Recurrence Noelia García-Fernández , Hada Macher, Amalia Rubio, Pilar Jiménez-Arriscado, Bernal-Bellido C, Bellido-Díaz ML, Gonzlo Suárez-Artacho, Juan M. Guerrero, Miguel A. Gómez-Bravo, Patrocinio Molinero .- 6 Unbiased Detection of Somatic Copy Number Aberrations in cfDNA of Lung Cancer Cases and High-risk Controls with Low Coverage Whole Genome sequencing Fiona Taylor, James Bradford, Penella J. Woll, Dawn Teare, Angela Cox.- 7 Liquid Profiling in Lung Cancer - Quantification of Extracellular miRNAs in Bronchial Lavage Bernd Schmidt, Grit Rehbein, Michael Fleischhacker.- 8 Screening of KRAS Mutation in Pre- and Post-surgery Serum of Patients Suffering from Colon Cancer by COLD-PCR HRM Elena Trujillo-Arribas, Hado C. Macher, Pilar Jiménez-Arriscado, Fernando de la Portilla, Patrocinio Molinero, Juan M. Guerrero, Amalia Rubio.- 9 Non-Dividing Cell Virtosomes Affect in vitro and in vivo Tumour Cell Replication Mariano Garcia-Arranz, Damian Garcia-Olmo, Luz Vega-Clemente, Maurice Stroun, Peter B. Gahan.- 10 Features of Circulating DNA Fragmentation in Blood of Healthy Females and Breast Cancer Patients Svetlana N. Tamkovich, Natalia A. Kirushina, Vladimir E. Voytsitskiy, Pavel P. Laktionov.- 11 Liquid Profiling of Circulating Nucleic Acids as a Novel Tool for the Management of Cancer Patients Stefan Holdenrieder.- Part II CNAPS in foetal medicine.- 12 Characterization of Human Pregnancy Specific Glycoprotein (PSG) Gene Copy Number Variations in Pre-eclampsia PatientsChia Lin Chang, Chia Yu Chang, Da Xian Lee, Po Jen Cheng.- 13 Non-Invasive Prenatal Diagnosis of Fetal-Maternal Platelet Incompatability by Cold High Resolution Melting Analysis Marta Ferro, Hada C. Macher, Pilar Noguerol, Pilar Jimenez-Arriscado, Patrocinio Molinero, Juan M. Guerrero, Amalia Rubio.- 14 Implementing Non-invasive Prenatal Diagnosis (NIPD) in a National Health Service Laboratory; from Dominant to Recessive Disorders Suzanne Drury, Sarah Mason, Fiona McKay, Kitty Lo, Christopher Boustred, Lucy Jenkins, Lyn S Chitty.- Part III BIOLOGY OF CNAPS.- 15 Comparative Analysis of Harmful Physical Factors Effects on the Genome of the Cell Irina N. Vasilyeva, Valery N. Zinkin, Vladimir G. Bespalov.- 16 Heterochromatic Tandem Repeats in the Extracellular DNA Olga I. Podgornaya, Irina N. Vasilyeva, Vladimir G. Bespalov.- 17 A Historical and Evolutionary Perspective on Circulating Nucleic Acids and Extracellular Vesicles: Circulating Nucleic Acids as Homeostatic Genetic Entities Janine Aucamp, Abel J Bronkhorst. Piet J Pretorius .- 18 Comparison of microRNA Content in Plasma and Urine Indicates the Existence of a TransrenalPassage of Selected microRNAs Eva Pazourkova, Sarka Pospisilova, Iveta Svobodova, Ales Horinek , Antonin Brisuda, Viktor Soukup, Jan Hrbacek, Otakar Capoun, Jaroslav Mares, Tomas Hanus, Marek Babjuk, Marie Korabecna.- 19 A Quantitative Assessment of Cell-free DNA Utilizing Several Housekeeping Genes: Measurements from Four Different Cell Lines Janine Aucamp, Abel Bronkhorst, Johannes F. Wentzel , Piet Pretorius.- 20 Oligodeoxynucleotide Analogues of Circulating DNA Inhibit dsRNA-induced Immune Response at the Early Stages of Signal Transduction Cascade in a Cell Type-dependent Manner Anna V. Cherepanova, Zhanna K. Nazarkina, Pavel P. Laktionov.- 21 GC-rich DNA Fragments and Oxidized Cell-free DNA Have Different Effects on NF-kB and NRF2 Signaling in MSC Vasilina A. Sergeeva, Svetlana V. Kostyuk, Elizaveta S. Ershova, Elena M. Malinovskaya, Tatiana D. Smirnova, Larisa V. Kameneva, Natalia N. Veiko.- 22 Evaluation of the State of Transplanted Liver health by Monitoring of Organ-specificgenomic Marker in Circulating DNA from Receptor Hada C. Macher, Gonzalo Suárez-Artacho, Pilar Jiménez-Arriscado, Sara Álvarez-Gómez, Noelia García-Fernández, Juan M. Guerrero, Patrocinio Molinero, Elena Trujillo-Arribas , Miguel A. Gómez-Bravo, Amalia Rubio.- 23 Vesicular and extra-vesicular RNAs of Human Blood Plasma Anna V. Savelyeva, Dmitriy N. Baryakin, Elena D. Chikova, Elena V. Kuligina, Vladimir A. Richter, Dmitriy V. Semenov.- 24 Artificial Analogues of Circulating Box C/D RNAs Induce strong Innate Immune Response and microRNA Activation in Human Adenocarcinoma Cells Grigory A. Stepanov, Julia A. Filippova, Anna A. Nushtaeva, Elena V. Kuligina, Olga A. Koval, Vladimir A. Richter, and Dmitriy V. Semenov.-25 Multiple Ways of cfDNA Reception and Following ROS Production in Endothelial Cells Anna Y. Alekseeva, Larisa V. Kameneva, Svetlana V. Kostyuk and Natalia N. Veiko.- 26 Protein Content of Circulating Nucleoprotein Complexes Svetlana N. Tamkovich,, Oleg S. Tutanov, Danil S. Serdukov, Maxim S. Belenikin, Anatoliy G. Shlikht, Natalia A. Kirushina, Tatiana G. Duzhak, Vladimir E. Voytsitskiy, Yuri P. Tsentalovich, Pavel P. Laktionov.- Part IV Methodology.- 27 Digital PCR of Genomic Rearrangements for Monitoring Circulating Tumour DNA Hongdo Do, Daniel Cameron, Ramyar MolaniaBibhusal Thapa, Gareth Rivalland, Paul L Mitchell, Carmel Murone, Thomas John, Tony Papenfuss, Alexander Dobrovic.- 28 MFast-SeqS as a Monitoring and Prescreening Tool for Tumour-specific Aneuploidy in Plasma DNA Methodological Variables in the Analysis of Cell-free DNA Jelena Belic, Marina Koch, Peter Ulz, Martina Auer, Teresa Gerhalter, Sumitra Mohan, Katja Fischereder, Edgar Petru, Thomas Bauernhofer5, Jochen B. Geigl, Michael R. Speicher, Ellen Heitzer.- 29 Methodological Variables in the Analysis of Cell-free DNA Abel J. Bronkhorst, Janine Aucamp, Piet J. Pretorius .- 30 Novel Technology for Enrichment of Biomolecules from Cell-Free Body Fluids and Subsequent DNA Sizing Vipulkumar Patel, Peter Celec, Magdalena Grunt, Heidi Schwarzenbach, Ingo Jenneckens, Timo Hillebrand.- 31 A Rapid and Sensitive Method for Detection of the T790M Mutation of EGFR in Plasma DNA Hideharu Kimura, Shingo Nishikawa, Hayato Koba, Taro Yoneda, Takashi Sone, Kazuo Kashara.- 32 Evaluation of Different Blood Collection Tubes and Blood Storage Conditions for the Preservation and Stability of Cell-free Circulating DNA for the Analysis of the MethylatedmSEPT9 Colorectal Cancer Screening Marker Jurgen Distler, ReimoTetzner, Gunter Weiss, Thomas König, Anne Schlegel, Michal Bagrowski.- 33 Purification of Circulating Cell-Free DNA from Plasma and Urine Using the Automated Large-Volume Extraction on the QIAsymphony SP Instrument Alexander Wolf, Katharina Beller, Sebastian Groemminger, Wera Hofmann, Matthias Sachse, Jana Fassunke.- 34 Detection and Quantification of KIT mutations in ctDNA by Plasma Safe-SeqS Johannes Fredebohm, Daniel Mehnert, Ann-Kathrin Löber, Frank Holtrup, Vanessa van Rahden, Philipp Angenendt, Frank Diehl.- Part V Ethics.- 35 Lost in translation? Ethical challenges of implementing a new diagnostic procedure Dagmar Schmitz.- Part VI Round-table.- 36 Academia meets Industry General Discussion between panel and audience.
