Biochemical and Structural Dynamics of the Cell Nucleus

Biochemical and Structural Dynamics of the Cell Nucleus is a collection of papers dealing with the biology of the cell nucleus. The collection describes the methods used in isolating and defining the chemistry and functional interactions of the nuclear components. Some papers also discuss the diversity of the roles that these components play in regulating cellular phenotypes, differentiation, and proliferation. One paper discusses the possible role for lamin A in muscle differentiations, where due to its presence and the appearance of A-type lamins during the differentiation of a number of cell types, lamin A or any of its isoelectric variants cannot specifically be responsible for the induction of muscle specific gene expression. The work of Loewinger and McKeon (1988) can show evidence of the role for lamin A in the differential expression of muscle-specific genes during the process of myogenesis if the investigator uses an alternative method. Several papers also discuss the dynamics of assembly and disassembly of the nuclear lamina and envelope, DNA- and RNA-binding proteins, as well as the nucleocytoplasmic transport. The collection can prove valuable to biochemists, cellular biologists, microbiologists, and molecular biologists.

Table of Contents:
Preface Section I Architecture and Structural Components of the Cell Nucleus The RNA-Containing Core Filaments of the Nuclear Matrix A Possible Role for Lamin A in Muscle Differentiation Molecular Cloning and Nuclear Localization of Lymphoid Membrane Skeletal Protein 4.1 Section II Dynamics of Assembly/Disassembly of Nuclear Lamina and Envelope Functional Analysis of Nuclear Lamins by Mutagenesis Nuclear Envelope Breakdown is Regulated by Calcium and CAM Kinase Calmodulin and Calmodulin Function in Liver Cell Nuclei Section III Proliferation/Differentiation-Dependent Nuclear Antigens Molecular Differentiation of a cDNA for Statin, a Non-Proliferating Cell-Specific Nuclear Protein, from Elongation Factor-1a Gene Monoclonal Antibodies Against Nuclear Antigens Associated with Proliferation and Differentiation of Bovine Esophageal Epithelium Gene Expression at the G1/S Boundary The Induction of c-Fos and c-Myc mRNA and the Expression of PCNA/Cyclin Dynamics of the Nucleus During Lymphocyte Activation Section IV DNA- and RNA-Binding Proteins Role of Topoisomerase I in Signal Transduction Factors Involved in the Spliceosome Assembly Pathway in Yeast Identification of a Carbohydrate Binding Protein in Heterogeneous Nuclear Ribonucleoprotein Complex (hnRNP) Section V Nucleocytoplasmic Transport An In Vitro Analysis of Nuclear Transport Analysis of Nuclear Envelope ATP-Binding Proteins A Dynamic Analysis of the Nuclear Pore Complex—The Intelligent Tunnel Index