Apoptosis Techniques and Protocols
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Apoptosis Techniques and Protocols

Apoptosis Techniques and Protocols

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International Edition


About the Book

In this revised and expanded second edition, seasoned experts describe in step-by-step detail their best state-of-the-art techniques for studying neuronal cell death. These readily reproducible methods solve a wide variety of research problems, including the detection of the key proteins involved in neuronal apoptosis (Bax protein, cytochrome c, and caspases), the direct assessment of the role of pro-apoptotic proteins in neurons by viral infections and microinjections, and the detection of pro-apoptotic proteins in situ. There are also hands-on methods for the study of apoptosis mechanisms in neuronal compartments, for studying synaptosis, and for establishing gene expression profiles in neurodegenerative brain tissues by using DNA microarrays.

Table of Contents:
Molecular Characterization of the Proapoptotic Protein Bax.- Assessing Cytochrome-c Release from Mitochondria.- Monitoring Caspases in Neuronal Cell Death.- HSV Amplicon Vectors in Neuronal Apoptosis Studies.- Microinjections to Study the Specific Role of Proapoptotic Proteins in Neurons.- Designing Microarray Experiments for Neurobiology.- Analysis of Gene Expression by Nylon Membrane cDNA Arrays.- Glyceraldehyde-3-phosphate Dehydrogenase as a Target for Antiapoptotic Drugs.- Analytical Approaches for Investigating Apoptosis and Other Biochemical Events in Compartmented Cultures of Sympathetic Neurons.- Detection and Analysis of Synaptosis.- In Situ Detection of Apoptotic Neurons.- Apoptotic and Oxidative Indicators in Alzheimer’s Disease.


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Product Details
  • ISBN-13: 9781617373060
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Edition: Revised edition
  • Language: English
  • Returnable: Y
  • ISBN-10: 1617373060
  • Publisher Date: 09 Nov 2010
  • Binding: Paperback
  • Height: 229 mm
  • No of Pages: 250
  • Width: 152 mm


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