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Stem Cell Research in Lab Animals

Stem Cell Research in Lab Animals


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About the Book

This book covers a comprehensive range of topics, with a particular focus on the latest information regarding mesenchymal stem cells (MSCs). It is divided into 17 chapters, which explore various aspects of stem cell technology, such as the different types of stem cells and their isolation from different animal sources. The book also includes information about the culture and characteristics of MSCs, as well as their osteoinductive potential. In addition, the book provides protocols for the isolation of MSCs from bone marrow, fat tissue, and embryonic stem cells. It also discusses the application of bioengineered stem cell technologies in skin wound healing, burn wound healing, critical bone defect healing, large bone defect healing, corneal wound healing, and liver regeneration. The book is intended to serve as a valuable resource for undergraduate and postgraduate research scholars, teachers, biologists, and clinicians involved in animal treatment and research. It features over 150 high-quality color photographs illustrating various aspects of stem cell isolation, culture, morphology, characteristics, and application in surgical interventions.

Table of Contents:
1. Stem Cells: Definition and Its Application 2. Isolation, Proliferation and Morphological Characteristics of Bone-marrow Derived Mesenchymal Stem Cells (BM-MSC) from Different Animal Species 3. Culture, Characterization and Differentiation Potential of Rat Bone Marrow Derived Mesenchymal Stem Cells 4. Isolation, Proliferation, Characterization of Rabbit Bone-Marrow Derived Mesenchymal Stem Cells (rBMSC) 5. In vitro Osteoinduction Potential of A Novel Silica Coated Hydroxyapatite Bioscaffold Seeded with Rabbit Mesenchymal Stem Cell 6. Implantation of MSC-Ceramic Construct for Repair of Critical Sized Bone Defect in Rabbit Model 7. Mesenchymal Stem Cells Seeded Bioceramic Construct for Bone Regeneration in Large Critical-size Bone Defect in Rabbit 8. Effect of Medical Grade Chitosan Powder with Mesenchymal Stem Cell for Full Thickness Skin Wound Healing in Rat Model 9. Evaluation of Mesenchymal Stem Cell Seeded Decellularized Porcine Intestine and Decellularized Cornea for the Repair of Corneal Defect in Rabbit Model 10. Effect of PDGF-beta Gene Activated Acellular Matrix and Stem Cell Transplantation on Full Thickness Burn Wound in Rat Model 11. Evaluation of Healing Potential of Chitosan and Porcine Small Intestinal Sub-Mucosa with Mesenchymal Stem Cells and Murine EGF for Full Thickness Burn Wound in Rat Model 12.Evaluation of Mesenchymal Stem Cells with Conditioned Media and m-EGF for Regeneration of Liver Tissue after Partial Hepatectomy in Rats 13. Role of Embryonic Stem cell (ESC)-Hydroxyapatite Construct with Growth Proteins for Osteogenesis in the Repair of Bone Defects in Rabbit Model 14. Protocol for Bone-marrow Derived MSC Isolation from Rat 15. Protocol for Fat Derived MSC Isolation from Rat 16. Protocol for Bone Marrow Derived MSC Isolation from Rabbit, Dog, Goat, Sheep and Pig 17. Protocol for Mesenchymal Stem cell Passaging References & Further Reading Colour Plates

About the Author :
Swapan Kumar Maiti, a graduate from Bidhan Chandra Krishi Viswa Vidyalaya (1984) followed by Masters (1987) and Doctorate (1990) in Veterinary Surgery from Indian Veterinary Research Institute. Dr Maiti joined ICAR- Indian Veterinary Research Institute as Scientist in 1993 and currently working as Principal Scientist in this Premier Indian Institute. Presently, Dr Maiti has more than 25 years of research experience in the field of Animal Oncology, Laparoscopic Surgery and Animal Stem Cells Biology. He is associated with more than 20 research projects and published more than 200 research and clinical papers in different peer reviewed International and National journals and presented more than 100 scientific papers in International and National Congress. Besides, he has authored two text-books, three manuals, two compendiums and contributed twenty chapters in different books. Dr Maiti has been invited as 'Speaker' in the 32nd, 33rd, 35th, 36th and 38th World Small Animal Veterinary Association (WSAVA) World Congress, held at Sydney (Australia); Dublin (Ireland); Geneva (Switzerland), Jeju (South Korea) and Auckland (New Zealand) respectively. He has also been invited as 'Speaker' in the 2nd Federation of Asian Small Animal Veterinary Association (FASAVA) Congress held at Bangkok (Thailand) in 2009. So far, he has visited more than 25 countries on different scientific assignments. He has been awarded prestigious fellowship from German Research Foundation (DFG), Germany for Post-Doctoral research work under International Bilateral Scientist Exchange Programme of Govt. of India. Dr Maiti deputed as 'Visiting Professor' at the University of Leipzig (2006), University of Köln (2010) and Technical University of Munich (2014), Germany. He has guided eight M.V.Sc, one M. Pharm and seven Ph.D students. He received many prestigious awards in his academic career including Prof A.K. Bhargava Gold Medal, Shri Ramlal Agrawal National Award (Gold Medal & Cash Prize); Prof S.J. Angelo Memorial Award, Indian Science Congress Centenary Best Paper (Certificate and Cash prize) and also nominated as 'International Expert' for assessment of teachers of Foreign University (Sudan) for three years. Dr Maiti is a 'Fellow' of National Academy of Veterinary Sciences, Indian Society for Veterinary Surgery and Indian Society for Advancement of Canine Practice for outstanding contribution in the field of Veterinary Science.


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Product Details
  • ISBN-13: 9789389130096
  • Publisher: New India Publishing Agency
  • Publisher Imprint: New India Publishing Agency
  • Height: 152 mm
  • No of Pages: 164
  • Spine Width: 229 mm
  • Width: 14 mm
  • ISBN-10: 9389130093
  • Publisher Date: 12 Jun 2020
  • Binding: Hardback
  • Language: English
  • Returnable: N
  • Weight: 521 gr


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