Essentials of Practical Microbiology

This book has several unique approaches which make it different from existing books. It is a clinical microbiology practical book. After reading this book, the students will have a bird’s eye view on how to diagnose infectious diseases clinically as well as how to go about laboratory investigations. It is the first microbiology book which is written in class-wise pattern according to MBBS practical class schedule. This will help the teacher know what to teach during a practical class, what to keep for demonstration during the practical class and also will give the teacher a holistic approach to make the yearly practical schedule. This will also help the students to know what to read during a practical class, what to write in the records and what to read during MBBS university practical examination. There is a separate chapter for university practical examination providing the pattern for various universities. This will help the students prepare for their practical examination. This will also help the teacher to modify or update the practical examination pattern. Each chapter starts with a problem-based exercise, i.e. clinical case scenario. The whole chapter isconcerned with solving the problem covering the important practical aspects of the topic. There are more than 600 images which will help students coordinate and read about the items demonstrated in the class with what is given in the practical book. Written in a concise, bulleted format and to-the-point text and simple and lucid language it includes several new practical class topics which are essential for MBBS graduates to learn, but are often missed.

Table of Contents:
Section 1: General Microbiology 1. Introduction to Microbiology Department 2. Microscopy 3. Simple Staining 4. Gram Staining 5. Special Staining 6. Bacterial Morphology and Growth 7. Sterilization and Disinfection 8. Culture Media 9. Culture Methods 10. Specimen Collection, Direct Microscopy, Processing for Culture and Motility Testing 11. Conventional Identification of Bacteria (Biochemical Reactions) 12. Automated Culture and Molecular Techniques 13. Antimicrobial Susceptibility Testing Section 2: Immunology 14. Precipitation and Agglutination 15. Enzyme-linked Immunosorbent Assay and Immunofluorescence Assay 16. Western Blot and Rapid Immunological Tests Section 3: Systematic Bacteriology 17. Staphylococcus 18. Streptococcus, Enterococcus and Pneumococcus 19. Neisseria 20. Corynebacterium and Bacillus 21. Anaerobes 22. Mycobacteria 23. Escherichia, Klebsiella and Proteus 24. Shigella and Salmonella 25. Vibrio and Pseudomonas 26. Haemophilus and Brucella 27. Syphilis, Leptospirosis and Scrub Typhus Section 4: Virology 28. Laboratory Diagnosis of Viral Diseases-I: Specimen Collection and Direct Detection of Viruses 29. Laboratory Diagnosis of Viral Diseases-II: Isolation, Serology and Molecular Methods 30. Herpesviruses 31. Influenza Measles and Rubella 32. Dengue, Rabies and Rotavirus 33. Hepatitis Viruses 34. Human Immunodeficiency Virus Section 5: Mycology 35. Laboratory Diagnosis of Fungal Diseases 36. Superficial, Subcutaneous and Deep Mycoses 37. Opportunistic Mycoses-I (Yeasts) 38. Opportunistic Mycoses-II (Molds) Section 6: Parasitology 39. Stool Examination 40. Entamoeba and Giardia 41. Leishmania and Trypanosoma 42. Plasmodium 43. Toxoplasma and Intestinal Coccidian Parasites 44. Cestodes 45. Trematodes 46. Intestinal Nematodes 47. Filarial Nematodes Section 7: Applied Microbiology 48. Bacteriology of Water, Air and Surface 49. Hospital-acquired Infection and Biomedical Waste 50. Vaccines 51. University MBBS Practical Examination Index