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Clonage et expression du gène de l'insuline humaine dans la levure de boulangerie

Clonage et expression du gène de l'insuline humaine dans la levure de boulangerie


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About the Book

Un monde exempt des ravages du diabète n'est pas seulement une vision admirable, mais une nécessité vitale. La découverte et l'isolement de l'insuline en 1921, par Banting et Best, ont ouvert la voie au développement d'un traitement qui a sauvé la vie de nombreux patients diabétiques. L'insuline a été la première protéine à être séquencée en 1951, marquant une étape importante dans la biologie moléculaire. En 1976, l'insuline est devenue le premier médicament recombinant - obtenu à l'aide de techniques de génie génétique nouvellement développées - à être utilisé pour le traitement chez l'homme. De nombreuses protéines, telles que l'insuline humaine, ne peuvent être obtenues à partir de leurs sources naturelles en quantités suffisantes en raison de leur faible abondance ou de la difficulté à les purifier par des méthodes conventionnelles. Une solution consiste à produire des protéines humaines recombinantes dans des systèmes cellulaires procaryotes hétérologues, tels que la levure, à l'aide de vecteurs d'expression puissants et adaptés. L'identification d'un système eucaryote simple dépend du niveau d'expression, de la qualité des biomolécules, de la sécurité, de la manipulation du système génétique et enfin du coût de production.


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Product Details
  • ISBN-13: 9786209210365
  • Publisher: Editions Notre Savoir
  • Publisher Imprint: Editions Notre Savoir
  • Height: 229 mm
  • No of Pages: 200
  • Returnable: N
  • Weight: 322 gr
  • ISBN-10: 6209210368
  • Publisher Date: 31 Oct 2025
  • Binding: Paperback
  • Language: French
  • Returnable: N
  • Spine Width: 12 mm
  • Width: 152 mm


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