About the Book
Technological advances have resulted in the development of several new biochemistry-based test procedures such as DNA micro-arrays, rapid sequencing and multiple applications of polymerase chain reaction. The limitations of such tests and the problems of standardization and validation related to their use for the detection of agents and toxins, diagnosis of human and animal diseases, and quality control of human and veterinary biological products must be considered. Practical examples of the use of these new technologies, but also the difficulties, limits and uncertainties encountered in their application need to be shared. This volume presents the proceedings of a conference in Saint-Malo, France, held to facilitate discussion between government authorities, industry, scientists and other users of these new techniques. Information on the principles of these novel methods, the need for standardization and validation, applications in diagnosis and surveillance, certainties and uncertainties in agent or toxin identification as well as examples of quality control of human and veterinary biological products were addressed. Conclusions and recommendations concerning the development and standardization of these new technologies were developed by the participants and are published in these proceedings. Professionals involved in biological standardization, diagnosis, and human and animal health programs should find this to be a very valuable book.
Table of Contents:
Principles of Novel Techniques: Tests Description and Potentialities; Experiences on the Application of Various Nucleic Acid Amplification Systems for the Improved Detection of Swine Vesicular Disease Virus (Abstract): Hakhverdyan, M.; Rasmussen, T.B.; Uttenthal, A.; Thoren, P.; Belak, S.; Detection of Animal Viruses Using Nucleic Acid Sequence-Based Amplification (NASBA): Lau, L.T.; Fung, Y.W.W.; Yu, A.C.H.; Standardisation, Validation and Overcoming the Problems of Molecular Diagnostic Methods; Development of a Framework for International Certification by OIE of Diagnostic Tests Validated as Fit for Purpose: Wright, P.; Edwards, S.; Diallo, A.; Jacobson, R.; Standardisation and Validation of Multiplex Real-Time PCR Systems (Abstract): Hoffmann, B.; Depner, K.; Beer, M.; Detection of Genetically Modified Plants: Limits and Possibilities (Abstract): Bertheau, Y.; Kobilinsky, A.; Davison, J.; Limits and Advantages of New Technologies in Criminology (Abstract): Schlenck, A.; Pascal, O.; Regulations in the United States Having an Impact on the Movement of Biological Materials (Abstract): Thomas, L.A.; Transportation of Reagents, Reference Materials and Samples: The International Perspective: Pearson, J.E.; Edwards, S.; Constraints on Transportation of Reagents, Reference Materials and Samples, Difficulties and Possible Solutions: a User's Perspective: Blanchard, P.C.; Application in Detection of Pathogens and Screening Programmes; A User's Inter-Laboratory Comparison of Broodfish Screening for Infectious Pancreatic Necrosis Virus Using Molecular and Conventional Diagnostic Methods: Storset, A.; Evensen, O.; Midtlyng, P.J.; Swine and Circovirus: Banks, M.; Grierson, S.; Tucker, D.; Bailey, M.; Donadeau, M.; Sargent, C.; King, D.; Mellencamp, M.; Does Real-Time RT-PCR for CSF Mark the Beginning of a Paradigm Shift in the Control of CSF? (Abstract): Depner, K.; Hoffmann, B.; Beer, M.; Certainties and Uncertainties of Agent or Toxin Identification by Molecular Methods; SARS/Avian Coronaviruses: Monceyron Jonassen, C.; Rapid Diagnosis of Avian Influenza (AI) and Assessment of Pathogenicity of Avian H5 and H7 Subtypes by Molecular Methods: Senne, D.A.; Pedersen, J.C.; Suarez, D.L.; Panigrahy, B.; Validation of a Real-Time RT-PCR Assay for Rapid and Specific Diagnosis of Classical Swine Fever Virus: Le Potier, M.F.; Le Dimna, M.; Kuntz-Simon, G.; Gougeard, S.; Mesplede, A.; BlueTongue: Characterization of Virus Types by Reverse Transcription-Polymerase Chain Reaction: Zientara, S.; Breard, E.; Sailleau, C.; Examples of Application in Quality Control of Human and Veterinary Vaccines; Antigen Mass Evaluation Before Formulation of FMD Inactivated Vaccines (Abstract): Dubourget, P.; Doel, T.R.; Development of an Inhibition ELISA Test for the Detection of Non-Capsid Polyprotein 3ABC in Viral Suspensions Destined for Inactivated Foot-and-Mouth Disease Vaccines: Bergmann, I.E.; Neitzert, E.; Malirat, V.; de Mendonca Campos, R.; Pulga, M.; Muratovik, R.; Quintino, D.; Morgado, J.C.; Oliveira, M.; de Lucca Neto, D.; Correlation Between Results of a Candidate in vitro Assay and Established in vivo Assays for Potency Determination of Inactived Newcastle Disease Vaccines (Abstract): Claassen, I.; Milne, C.; Maas, R.; Risk Assessment in New and Conventional Vaccines: Dory, D.; Gravier, R.; Jestin, A.; Conclusions and Recommendations; Conclusions; Constraints; Recommendations and Solutions.
