Buy Protein Structure Analysis by Brigitte Wittmann-Liebold
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Home > Mathematics and Science Textbooks > Biology, life sciences > Cellular biology (cytology) > Protein Structure Analysis: Preparation, Characterization, and Microsequencing(Springer Lab Manuals)
36%
Protein Structure Analysis: Preparation, Characterization, and Microsequencing(Springer Lab Manuals)

Protein Structure Analysis: Preparation, Characterization, and Microsequencing(Springer Lab Manuals)


     0     
5
4
3
2
1



Available


X
About the Book

"Protein Structure Analysis - Preparation and Characterization" is a compilation of practical approaches to the structural analysis of proteins and peptides. Here, about 20 authors describe and comment on techniques for sensitive protein purification and analysis. These methods are used worldwide in biochemical and biotechnical research currently being carried out in pharmaceu­ tical and biomedical laboratories or protein sequencing facilities. The chapters have been written by scientists with extensive ex­ perience in these fields, and the practical parts are well documen­ ted so that the reader should be able to easily reproduce the described techniques. The methods compiled in this book were demonstrated in student courses and in the EMBO Practical Course on "Microsequence Analysis of Proteins" held in Berlin September 10-15, 1995. The topics also derived from a FEBS Workshop, held in Halkidiki, Thessaloniki, Greece, in April, 1995. Most of the authors participated in these courses as lecturers and tutors and made these courses extremely lively and successful. Since polypeptides greatly vary depending on their specific structure and function, strategies for their structural analysis must for the most part be adapted to each individual protein. Therefore, advantages and limitations of the experimen­ tal approaches are discussed here critically, so that the reader becomes familiar with problems that might be encountered.

Table of Contents:
I Separation of Proteins.- 1 Purification of Proteins for Sequencing.- 2 Affinity Chromatography of Proteins.- 3 Protein Purification by Aqueous Two-Phase Systems.- 4 Rapid HPLC Separation of Proteins, Peptides, and Amino Acids Using Short Columns Filled with Nonporous Silica-Based Particles.- II Separation of Peptides.- 5 Enzymatic and Chemical Cleavages of Proteins.- 6 Separation of Peptides Using HPLC and TLC Fingerprints.- 7 Microseparation of In Situ Digested Peptides.- 8 Isolation of Peptides for Microsequencing by In-Gel Proteolytic Digestion.- III Manual and Automated Microsequencing Methods.- 9 Automated Microsequencing: Introduction and Overview.- 10 A Manual Method for Protein Sequence Analysis Using the DABITC/PITC Method.- 11 Solid-Phase Sequencing of Peptides and Proteins.- 12 Sequence Analysis of the NH2-Terminally Blocked Proteins Immobilized on PVDF Membranes from Polyacrylamide Gels.- IV Electrophoresis and Blotting of Proteins.- 13 Two-Dimensional Electrophoresis.- 14 Semi-Dry Blotting onto Hydrophobic Membranes.- V Analysis of Amino Acids.- 15 Highly Sensitive Amino Acid Analysis.- 16 Quantitative Analysis of D- and L-Amino Acids by HPLC.- VI Identification of Cysteine Residues and Lipids in Proteins.- 17 Identification of Cysteine Residues and Disulfide Bonds in Proteins.- 18 Lipid Modifications of Proteins.- VII Mass Spectrometry in Peptide and Protein Analysis.- 19 Mass Spectrometry in Peptide and Protein Sequence Analysis.- 20 Carboxy-Terminal Sequencing by Combining Carboxypeptidase Y and P Digestion with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry.- 21 Crystallization of Biological Macromolecules.

Review :
"The most advanced and sensitive methods have been selected for this manual. The presented procedures are well established and have been found useful in many laboratories." Bioscope


Best Sellers


Product Details
  • ISBN-13: 9783642477652
  • Publisher: Springer-Verlag Berlin and Heidelberg GmbH & Co. KG
  • Publisher Imprint: Springer-Verlag Berlin and Heidelberg GmbH & Co. K
  • Height: 235 mm
  • No of Pages: 316
  • Returnable: Y
  • Sub Title: Preparation, Characterization, and Microsequencing
  • ISBN-10: 3642477658
  • Publisher Date: 07 Nov 2012
  • Binding: Paperback
  • Language: English
  • Returnable: Y
  • Series Title: Springer Lab Manuals
  • Width: 155 mm


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
Protein Structure Analysis: Preparation, Characterization, and Microsequencing(Springer Lab Manuals)
Springer-Verlag Berlin and Heidelberg GmbH & Co. KG -
Protein Structure Analysis: Preparation, Characterization, and Microsequencing(Springer Lab Manuals)
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

Protein Structure Analysis: Preparation, Characterization, and Microsequencing(Springer Lab Manuals)

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept


    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!