Foreign Gene Expression in Fission Yeast: Schizosaccharomyces pombe
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Home > Mathematics and Science Textbooks > Biology, life sciences > Cellular biology (cytology) > Foreign Gene Expression in Fission Yeast: Schizosaccharomyces pombe: (Biotechnology Intelligence Unit)
Foreign Gene Expression in Fission Yeast: Schizosaccharomyces pombe: (Biotechnology Intelligence Unit)

Foreign Gene Expression in Fission Yeast: Schizosaccharomyces pombe: (Biotechnology Intelligence Unit)


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About the Book

Recombinant proteins, produced in cells or micro-organisms, play an important role in basic research, medical science and the biotechnology industry. Many foreign gene expression systems using mammalian cells, E. coli, insect cells, yeast and other organisms as hosts have been developed. Current focus in the field is on schizocsaccharomyces pombe as a host, as it is expected to be profitable for expressing foreign genes. This work describes the use of schizocsaccharomyces pombe in basic research and industrial applications.

Table of Contents:
1. Fission Yeast Schizosaccharomycespombe: An Attractive Host for Heterologous Protein Production.- 2. Constitution of Expression Vectors.- 3. Exploiting the Thiamine Biosynthetic Gene nmtl for Regulated Expression of Heterologous DNAs in Fission Yeast.- 4. Development of Constitutive Expression Vector for the Highest Production Level of Heterologous Proteins in Fission Yeast Schizosaccharomycespombe.- 5. Overexpression of Proteins with Seven Transmembrane Spanning Segments.- 6. Targeting and Isolation of Tagged Membrane Proteins.- 7. Expression of a Human Cytochrome P450 Form in Schizosaccharomyces pombe: Comparison with Expression in Saccharomyces cerevisiae.- 8. Human Papillomavirus Capsid Protein-pREP in Schizosaccharomyces pombe: Efficient Assembly of the Viral Capsid Protein in S. pombe and S. cerevisiae.- 9. Expression, Purification, and Analyses of Cell-Cycle Regulatory Proteins in S. pombe.- 10. Expression and Secretion of Mammalian Proteins in Schizosaccharomyces pombe.- 11. Secretion of Human Interleukin-6 Using the P-Factor Secretion Signal in Schizosaccharomyces pombe.- Color Figures.



Product Details
  • ISBN-13: 9783540632702
  • Publisher: Springer-Verlag Berlin and Heidelberg GmbH & Co. KG
  • Publisher Imprint: Springer-Verlag Berlin and Heidelberg GmbH & Co. K
  • Height: 297 mm
  • No of Pages: 179
  • Series Title: Biotechnology Intelligence Unit
  • ISBN-10: 3540632700
  • Publisher Date: 17 Oct 1997
  • Binding: Hardback
  • Language: English
  • Returnable: Y
  • Width: 210 mm


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