An indispensable guide to a novel, revolutionary fluorescence microscopy technique!
Light sheet-based fluorescence microscopy has revolutionized microscopy, since it allows scientists to perform experiments in an entirely different manner and to record data that had not been accessible before. With contributions from noted experts in the fields of physics, biology, and computer science, Light Sheet Fluorescence Microscopy is a unique guide that offers a practical approach to the subject, including information on the basics of light sheet fluorescence microscopy, instrumentation, applications, sample preparation, and data analysis.
Comprehensive in scope, the book is filled with the cutting-edge methods as well as valuable insider tips. Grounded in real-world applications, the book includes chapters from major manufacturers that explores their recent systems and developments. In addition, the book hightlights a discussion of a “do-it-yourself” light sheet microscope, making the technique affordable for every laboratory. This important book:
- Serves as an easy-to-understand introduction to light sheet-based fluroescence
- Includes numerous tips and tricks for advanced practitioners
- Provides in-depth information on hardware and software solutions for a straightforward implementation of light sheet fluorescence microscopy in the lab
- Includes chapters from the major manufacturers including Zeiss, Leica, Lavision Biotech, Phase View, and Asi
Aimed at cell biologists, biophysicists, developmental biologists, and neurobiologists, Light Sheet-based Fluorescence Microscopy offers a comprehensive overview of the and the most recent applications of this microscopy technique.
Table of Contents:
LIGHT SHEET MICROSCOPY HARDWARE
Physics of Light Sheet-based Fluorescence Microscopes (e.g. Light Sheet formation, Optics, Detectors)
Overview of the Flavors of Light Sheet Microscopy (e.g. Arrangements of Optical Axes, Schematics, Commercial Set-ups, Extensions to Other Modalities)
Driving the Microscope and Open Access Electronics (e.g. Software to Pilot Light Sheet Microscopes (MicroManager), Arduino Solutions)
"Do It Yourself" Light Sheet based Microscope (e.g. OpenSPIM/OpenSPIN/LegoSPIM)
SAMPLE MOUNTING FOR LIGHT SHEET MICRSOCOPY
Sample Handling and Preparation (Including Sample Chamber Designs, Sample Positioning)
Clearing of Biological Tissues For Light Sheet Microscopy
Operation of Light Sheet-based Fluorescence Microscopes (Including In Multi-user Environment)
LIGHT SHEET MICRSOCOPY SOFTWARE
Image Processing of Multi-view Data (Registration, Fusion, and Deconvolution))
Visualization of Big Image Data (e.g. Local and Remote Viewing, 3D Rendering, Annotation)
Information Technology of Big Data Handling (e.g. Compression, Storage, Transfer)
Extracting Information From Light Sheet Datasets (e.g. Image Analysis Such As Atlas Registration, Segmentation, Tracking)
LIGHT SHEET MICROSCOPY APPLICATIONS
Applications In Developmental Biology
Applications In Cell Biology
Applications In Neurobiology
Applications In Biodiversity
LIGHT SHEET MICROSCOPY IMPLEMENTATIONS
Imaging Platform Integration
Laboratory Integration
IT Supports
About the Author :
Emmanuel Reynaud, PhD, is Stokes Lecturer in Biology at University College Dublin, Ireland.
Pavel Tomancak, PhD, is Senior Permanent Research Group Leader at the Max Planck Institute of Molecular Cell Biology and Genetics in Dresden, Germany.
